Qihui Pua, Ryan Spoonera, Lisa A. DeLouisea,b,* Supplementary Data Identifying Drug Resistant Cancer Cells Using Microbubble Well Arrays   Qihui Pua, Ryan Spoonera, Lisa A. DeLouisea,b,* aDepartment of Biomedical Engineering, University of Rochester, Rochester, NY bDepartment of Dermatology, University of Rochester Medical Center, Rochester, NY *Email correspondence: lisa_delouise@urmc.rochester.edu

Supplementary Figure S1 B Supplementary Fig. S1 (A) Primary human keratinocytes, derived from human skin, cultured in KGM media on 2D tissue culture plastic (TCP). (B) SCC cells, derived from a human tumor, cultured in KGM media on 2D tissue culture plastic. At low density both cell types grow as adherent cells with little tendency to cluster or exhibiting distinguishable morphological differences. SCC sphere cells do not form on TCP. Images taken at 10X magnification.

Supplementary Figure S2 Supplementary Fig. S2 SCC cytotoxicity results. SCC cells were culture with different concentrations of cisplatin for 48 hr. The common MTT (measure of mitochrondial enzyme activity) and cell titer glo (CTG, measure of ATP) assays were used to measure cell viability and results were combined (N=4). Curve fitting the above data gave an LD50 ~5 µM.

Supplementary Figure S3 BF CalceinAm CD44 CD133 Figure S3 Immunofluorescence images showing SCC spheres express common CD44 and CD133 stem cell markers. Scale bar 400 µm.

Supplementary Figure S4 Figure S4 Image of SCC tumor from patient used in this work prior to cellularization.