Metabolic reprogramming results in chemosensitivity in IDH1 mutant cells. Metabolic reprogramming results in chemosensitivity in IDH1 mutant cells. A,

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Metabolic reprogramming results in chemosensitivity in IDH1 mutant cells. Metabolic reprogramming results in chemosensitivity in IDH1 mutant cells. A, Seahorse XF Cell Mito Stress analysis showed alteration in oxidative metabolism in IDH1 mutant cells (n = 4). B, Quantification of baseline oxidative metabolism in IDH1 mutant cells (n = 4; **, P < 0.01). C, Quantification of maximum oxidative metabolism in IDH1 mutant cells (n = 4; **, P < 0.01). D, Quantification of D-2-HG in IDH1 mutant cells. AGI-5198 treatment (1 μmol/L) was used to determine specific activity from IDH1 mutant enzyme (n = 3; **, P < 0.01). E, Cell viability assessment of U87 MG cells in the presence of TMZ and dimethyl-2-HG (n = 3). F, Cell viability measurement of HT-1080 cells with the presence of TMZ and dimethyl-α-KG (n = 3; *, P < 0.05 as compared with DMSO group; #, P < 0.05 as compared with TMZ group). G, Cell viability assessment of HT-1080 cells with stable expression of IDH1 shRNA (n = 3; *, P < 0.05 as compared with 0 μmol/L TMZ; #, P < 0.05 and ##, P < 0.01 as compared with scramble RNA). H, Quantitative PCR (top) and Western blot analysis (bottom) for IDH1 expression in HT-1080 cells (n = 3). Yanxin Lu et al. Cancer Res 2017;77:1709-1718 ©2017 by American Association for Cancer Research