OVERVIEW OF HYDROGEN PRODUCTION Steam reforming - Natural gas /Coal Thermo chemical metods-Using thermocyclers France , Germany ,Japan ,USA- countries produces hydrogen 3. Biohydrogen

LIMITATION Co production of oxygen Presence of nitrogenase ( oxygen sensitive enzymes) Continuous production of hydrogen Unavailability of commercial production methods.

Photosynthetic bacterial hydrogen production Cyanobacterial hydrogen production - Atmospheric condition Photosynthetic bacterial hydrogen production -media composition , identification - light ,substrate, atmospheric condition. Nitrogen Argon Methane CO2

Co- cultivation of Cyanobacteria and photosynthetic bacteria Designing photo-bioreactor for large scale continuous production

Light duration - 24 hours [ 18/6] total volume - approx. 13 ml vials Conditions : Temperature - 30°C Light Intensity - 400 lux Light duration - 24 hours [ 18/6] total volume - approx. 13 ml vials culture volume - 5 ml

NITROGEN ATMOSPHERE

ARGON ATMOSPHERE

METHANE ATMOSPHERE

Pink color Yellow color Dark brown color Green color Identification for genus and species.

Solution I Solution II SolutionIII g/L Trace metal mix mg/L Potassium di-hydrogen phosphate 0.33 mg/L Zinc sulfate 10 Ferrous sulfate 200 Ammonium chloride 0.5 Manganese chloride 3 Magnesium chloride 3 NOTE 1ml of Solution II & 0.5ml of Solution III were added to the media after autoclaved. Boric acid 30 Sodium chloride 25 Cobaltous chloride 20 Calcium chloride 0.05 Cupric chloride 1 Sodium succinate 1.0 Nickel chloride 2 Yeast extract 0.02 Sodium molybdate 3 Agar 25 pH 3-4 pH 7

1.Purple non sulfur bacteria 2. Green sulfur bacteria