Reactive oxygen species and membrane permeability assay. Reactive oxygen species and membrane permeability assay. Cells were grown to the early linear phase in medium A+ or 12.5% GBF with the appropriate treatment under conditions of continuous illumination (200 μmol photons m−2·s−1) with 1% CO2 at 37°C. Fluorescence values were normalized to OD730. The values represent means ± SD of data from biological triplicates. Travis C. Korosh et al. mSphere 2018;3:e00538-17 Copyright © 2018 Korosh et al.