a b Suppl. Fig. 1

Suppl. Fig. 2 mock-infected CVB3 MOI 1 0,4% 5,1% CVB3 MOI 5 20,3% 34,2% Suppl. Fig. 2

Suppl. 1 Figure Legends Suppl. Fig. 1 The protein detected by the antibody Cox mAb 31A2 (see Fig. 3c) was analyzed by peptide mass fingerprinting (MALDI-TOF) with either trypsin (a) or chymotrypsin (b) and was identified as VP1 from CVB3.   Suppl. Fig. 2 FACS analysis of HeLa cells mock-infected or infected with different MOI of CVB3 (1; 5; 10) for 5 h. Cells were stained with Cox mAb 31A2 and Alexa488 goat anti-mouse antibody was used as secondary antibody. The antibody Cox mAb 31A2 gives a specific, MOI dependent signal with nearly no background staining. The experiment was repeated 3 times with comparable results. Improving diagnostic approaches for a highly specific detection of coxsackievirus B3 in the heart Virchows Archiv Nicole Ettischer-Schmid, Andrea Normann, Martina Sauter, Lisa Kraft, Hubert Kalbacher, Reinhard Kandolf, Bertram Flehmig, Karin Klingel K. Klingel, Institute for Pathology, Dept. of Molecular Pathology, University Hospital Tuebingen, Germany, E-mail: karin.klingel@med.uni-tuebingen.de