Expression of KIR2DL1 on the entire NK cell population: a possible novel immunodeficiency syndrome by Roi Gazit, Ben-Zion Garty, Yehudith Monselise, Vered Hoffer, Yaron Finkelstein, Gal Markel, Gil Katz, Jacob Hanna, Hagit Achdout, Raizy Gruda, Tsufit Gonen-Gross, and Ofer Mandelboim Blood Volume 103(5):1965-1966 March 1, 2004 ©2004 by American Society of Hematology

KIR2DL1 is expressed on all NK cells derived from the patient. KIR2DL1 is expressed on all NK cells derived from the patient. (A) Bulk NK cultures derived from the patient were incubated with 721.221 targets (221; □), or with 721.221 transfected with HLA-Cw4 (Cw4; ▪), at the indicated effector-target (E/T) ratios. (B) Fluorescence-activated cell sorter (FACS) analysis for KIR2DL1 expression of bulk NK cultures derived from the patient (left) and a representative healthy donor (right) using the EB6 mAb. Staining (open histograms) is shown overlaid on background (shaded histograms). The NK cells were incubated for one hour with or without anti-KIR2DL1 mAb HP3E4 or the anti-CD99 mAb 12E7 and then tested against the various targets as in panel A. Shown is a representative NK clone (C) and NK bulk cultures (D), both derived from the patient. Roi Gazit et al. Blood 2004;103:1965-1966 ©2004 by American Society of Hematology