Arterioscler Thromb Vasc Biol

Slides:



Advertisements
Similar presentations
Qian Lingbo Protein Kinase C–Dependent Increase in Reactive Oxygen Species Production in Vascular Tissues of Diabetes: Role of Vascular NAD(P)H.
Advertisements

From: Transient Hyperglycemia Affects the Extent of Ischemia-Reperfusion-induced Renal Injury in Rats Anesthes. 2008;108(3): doi: /ALN.0b013e318164cff8.
From: Lentiviral Vector-Mediated PAX6 Overexpression Promotes Growth and Inhibits Apoptosis of Human Retinoblastoma Cells Invest. Ophthalmol. Vis. Sci..
Ursolic acid from the Chinese herb Danshen (Salvia miltiorrhiza L
Arterioscler Thromb Vasc Biol
Role of Jagged1 in Arterial Lesions After Vascular Injury
by Hideto Sano, Kohei Hosokawa, Hiroyasu Kidoya, and Nobuyuki Takakura
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
by Johannes B. K. Schwarz, Nicolas Langwieser, Nicole N
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Regulation of Hypoxia-Inducible Genes by PGC-1α
Vascular PPARδ Protects Against Stroke-Induced Brain Injury
Arterioscler Thromb Vasc Biol
New LINE(s) of Evidence for Genetic Regulation of Platelets
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Effect of Early Particulate Air Pollution Exposure on Obesity in Mice
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Bone Morphogenic Protein 4 Produced in Endothelial Cells by Oscillatory Shear Stress Induces Monocyte Adhesion by Stimulating Reactive Oxygen Species Production.
Assessment of Unstable Atherosclerosis in Mice
Arterioscler Thromb Vasc Biol
Role for Hyaluronan Synthase 3 in the Response to Vascular Injury
Arterioscler Thromb Vasc Biol
Dynamic Modulation of Interendothelial Gap Junctional Communication by 11,12-Epoxyeicosatrienoic Acid by Rüdiger Popp, Ralf P. Brandes, Gregor Ott, Rudi.
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
HIV Protease Inhibitors Stimulate Hepatic Triglyceride Synthesis
by Karen Reue, Robert D. Cohen, and Michael C. Schotz
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Arterioscler Thromb Vasc Biol
Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells  Wen-Jane Lee, PhD, Hsiu-Chung.
Volume 68, Issue 6, Pages (December 2005)
IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.
J. Chen, W. Chen, M. Zhu, Y. Zhu, H. Yin, Z. Tan 
Antiangiogenic antithrombin down-regulates the expression of the proangiogenic heparan sulfate proteoglycan, perlecan, in endothelial cells by Weiqing.
Mechanisms of oxidative stress in human aortic aneurysms — Association with clinical risk factors for atherosclerosis and disease severity  Bartłomiej.
Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells 
Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells  Wen-Jane Lee, PhD, Hsiu-Chung.
Wei Zhou, MD, Hong Chai, MD, PhD, Peter H. Lin, MD, Alan B
Volume 76, Issue 11, Pages (December 2009)
Volume 69, Issue 4, Pages (February 2006)
Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor κB target genes and induction of apoptosis in chronic.
Edward A. Black, Tomasz J. Guzik, MD, Nick E. J
Vascular endothelial growth factor stimulates protein kinase CβII expression in chronic lymphocytic leukemia cells by Simon T. Abrams, Benjamin R. B. Brown,
Volume 78, Issue 9, Pages (November 2010)
Volume 68, Issue 6, Pages (December 2005)
Characterization of endothelin receptors in human varicose veins
NADPH Oxidase 1 Overexpression Enhances Invasion via Matrix Metalloproteinase-2 and Epithelial–Mesenchymal Transition in Melanoma Cells  Feng Liu, Angela.
Eberhard Schulz, and Thomas Münzel JACC 2008;52:
Volume 80, Issue 1, Pages (July 2011)
Romain Debret, Richard R
Collagen Synthesis Is Suppressed in Dermal Fibroblasts by the Human Antimicrobial Peptide LL-37  Hyun Jeong Park, Dae Ho Cho, Hee Jung Kim, Jun Young.
Volume 70, Issue 5, Pages (September 2006)
John M. Lamar, Vandana Iyer, C. Michael DiPersio 
Mechanisms of Hypoxic Regulation of Plasminogen Activator Inhibitor-1 Gene Expression in Keloid Fibroblasts  Qunzhou Zhang, Yidi Wu, David K. Ann, Diana.
Figure 2 LMNB1 mRNA expression
Analysis of mRNA and protein levels of Loricrin, COMP, CXCL9, KRT19, and CYP 3A5 genes in OSFs compared with normal controls. Analysis of mRNA and protein.
Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.
Presentation transcript:

Arterioscler Thromb Vasc Biol Systemic Regulation of Vascular NAD(P)H Oxidase Activity and Nox Isoform Expression in Human Arteries and Veins by Tomasz J. Guzik, Jerzy Sadowski, Boguslaw Kapelak, Andrzej Jopek, Pawel Rudzinski, Ravi Pillai, Richard Korbut, and Keith M. Channon Arterioscler Thromb Vasc Biol Volume 24(9):1614-1620 September 1, 2004 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Sources of vascular superoxide generation in human saphenous veins and mammary arteries. Figure 1. Sources of vascular superoxide generation in human saphenous veins and mammary arteries. Superoxide production was determined by lucigenin-enhanced chemiluminescence (20 μmol/L lucigenin) in saphenous vein (HSV) and internal mammary artery (IMA) segments (n=21). Vessels were incubated for 30 minutes before and during superoxide determination with oxidase inhibitors: diphenyleneiodonium (50 μmol/L), apocynin (500 μmol/L), oxypurinol(100 μmol/L), rotenone (100 μmol/L), or l-NMMA (100 μmol/L). Oxygen consumption was measured to ensure that mitochondrial respiration was inhibited by rotenone. Superoxide generation was expressed as RLU/mg dry weight (mean±SEM). *P<0.05 versus basal; **P<0.01 versus basal; ¶P<0.01 versus HSV. Tomasz J. Guzik et al. Arterioscler Thromb Vasc Biol. 2004;24:1614-1620 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. NAD(P)H oxidase protein subunit levels and activity in human saphenous veins and mammary arteries. Figure 2. NAD(P)H oxidase protein subunit levels and activity in human saphenous veins and mammary arteries. A, Western blots showing NAD(P)H oxidase subunits, p22phox, p67phox, and p47phox in saphenous vein (HSV) and IMA. α-Actin band shows equal loading of protein. B, Densitometric analysis of bands in HSV and IMA (mean±SEM; paired n=8), expressed in relation to actin. C, Superoxide production measured from vascular homogenates in response to 100 μmol/L NADPH or NADH measured using SOD-inhibitable ferricytochrome c reduction assay. Bars show mean±SEM (n=10 patients). *P<0.01 versus HSV. Tomasz J. Guzik et al. Arterioscler Thromb Vasc Biol. 2004;24:1614-1620 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Relationships between human arteries and veins in vascular superoxide production and endothelial function. Figure 3. Relationships between human arteries and veins in vascular superoxide production and endothelial function. Basal superoxide (O2−) production (A), maximal NAD(P)H oxidase activity (B), and endothelial function (C) were determined in paired segments of IMA and HSV. Superoxide production and NAD(P)H oxidase activity in intact vascular rings were measured using lucigenin-enhanced chemiluminescence (5 μmol/L) and endothelial function was assessed using maximal acetylcholine-dependent vasorelaxations (ACh max); n=31 patients. Tomasz J. Guzik et al. Arterioscler Thromb Vasc Biol. 2004;24:1614-1620 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Molecular composition of NAD(P)H oxidases in saphenous veins and mammary arteries. Figure 4. Molecular composition of NAD(P)H oxidases in saphenous veins and mammary arteries. A, RT-PCR of NADPH oxidase subunits of HSV and IMA. Equivalent RNA amounts (20 ng) were used. Inflammatory cell content was assessed by MCSFR mRNA expression. THP-1 RNA (nox2; p22phox; MCSFR) or hMEC RNA (nox1, nox4) were used as positive control. B, Quantitative RT-PCR of NADPH oxidase subunits of HSV and IMA (n=10 pairs). Results were normalized to mRNA levels of the housekeeping gene MLN-51. *P<0.05 versus HSV. Tomasz J. Guzik et al. Arterioscler Thromb Vasc Biol. 2004;24:1614-1620 Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Relationships between NAD(P)H oxidase subunit mRNA expression in arteries and veins. Figure 5. Relationships between NAD(P)H oxidase subunit mRNA expression in arteries and veins. Quantitative RT-PCR of NADPH oxidase subunit mRNA (p22phox, nox2, and nox4) of HSV and IMA (n=10 patients) was performed as described in Methods; 20 ng of total RNA was used and results were normalized to expression of the housekeeping gene MLN-51. Relationships were analyzed using Pearson correlation analysis. Tomasz J. Guzik et al. Arterioscler Thromb Vasc Biol. 2004;24:1614-1620 Copyright © American Heart Association, Inc. All rights reserved.