VUMC Soil Worm Activity Monitor Mohd Fakhrurrazi Mohd Salleh (EE) Suhaili Harun (EE) Amani Rafie (CompE) Saiful Azlan Adanan (BME) March 31, 2005

Progress Turned in the proposal for hardware and software request Ordered the computer and the capture card Tried to convert the 1-hour video to analog-avi format Apply the sliding window method in image processing

Operational Concept Video Capturing Image Processing

Video Capturing Operational Concept Turn on power of all equipment (microscope, computer, camera Insert 40mL of distilled water into a 6mm-diameter well Load the capture card software Put under microscope Put a worm in the well using a pick Image Processing Separated the video into frames using Windows Movie Maker Using Matlab Import into Matlab Subsampling images Color separation for image segmentation Analysis of the difference between frames Determine the velocity of the worm based on its centroid Record video of the worm using the camera software AVI-analog video file format to image processing

Image Processing Operational Concept Load AVI-analog video into MatLab Downsample each frame by 50% Color Separation Filter background noise by taking difference between frames Detrend each data plot Plot the components of the centroid x-component Determine worm centroid in each frame y-component Image Processing Separated the video into frames using Windows Movie Maker Using Matlab Import into Matlab Subsampling images Color separation for image segmentation Analysis of the difference between frames Determine the velocity of the worm based on its centroid Apply FFT Numerical and graphical results Determine the frequency at the peak Add both components Determine sinusoidal cycle

Detrend Detrending is the statistical or mathematical operation of removing trend from the series It is often applied to remove a feature thought to distort or obscure the relationships of interest In our case, the feature we are removing is the translation of the worm

Adding the velocity of both x and y components Amplitude Frequency Calculated Frequency = 2.00 Hz

Frequency vs. time

Future Work Expecting to receive the computer and the capture card Installing the capture card and software into computer Process the one-hour worm video