Cytokine-induced rapid ICAM-1 expression on human ASM promotes adhesion of activated T lymphocytes. Cytokine-induced rapid ICAM-1 expression on human ASM promotes adhesion of activated T lymphocytes. A, ASM cells were incubated for the indicated time with 10 ng/ml TNFα. In other experiments, cells were pretreated with CHX for 30 min and then stimulated with TNFα for 4 h. ICAM-1 expression was analyzed by flow cytometry as described in Materials and Methods. Values shown are mean ± S.E. (n = 4 separate experiments), *P < .05, **P < .01 compared with untreated cells, and#P < .01 compared with cells treated with TNFα. B, T cells were cultured without (unstim.) or with phorbol-12,13-dibutyrate and ionomycin (stim.) and pulsed with3H-labeled thymidine (1 μCi/106 cells) for 16 h. ASM cells were pretreated with isotype matched antibodies in control media (unstim.) or with anti-ICAM-1 antibodies (10 μg/ml) for 45 min and then stimulated with TNFα for an additional 4 h. Adhesion assays were performed as described inMaterials and Methods. Each condition was performed in triplicate (n = 3;#P < .01 compared with untreated ASM cells, and *P < .05 compared with ASM cells treated with TNFα). Yassine Amrani et al. Mol Pharmacol 2000;58:237-245 The American Society for Pharmacology and Experimental Therapeutics