HMGA2 is a SUMOylation target.

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Presentation transcript:

HMGA2 is a SUMOylation target. HMGA2 is a SUMOylation target. A, HMGA2 is SUMOylated in vitro. In vitro SUMOylation reaction was done as described in Materials and Methods, and analyzed with the indicated antibodies. B, HMGA2 is modified by EGFP-SUMO-1, but not by EGFP-SUMO-1aa, in vivo. COS-1 cells were transfected with HMGA2, and EGFP-SUMO-1/EGFP-SUMO-1aa, as indicated. Cells were lysed and analyzed by immunoblotting using an anti-HA antibody to detect both HMGA2 and SUMOylated HMGA2. An anti-GFP antibody was used to detect EGFP-SUMO-1 and EGFP-SUMO-1aa. C, the effect of ΔAxam on HMGA2 SUMOylation. Whole-cell lysates of COS-1 cells expressing the indicated protein were probed with an anti-HA antibody. D, mapping the SUMO-1 modification site of HMGA2. The functional domains and mutation sites were schematically illustrated as in the top panel. Hatched boxes, the AT-hook DNA-binding domain. COS-1 cells were transfected with HMGA2, HMGA2(4P/A), and HMGA2(2K/R), respectively. * in C and D, nonspecific species while using a particular lot of an anti-HA antibody. Relative levels of SUMO-HMGA2 in C and D, after normalization, are indicated as italic. Xuefei Cao et al. Mol Cancer Ther 2008;7:923-934 ©2008 by American Association for Cancer Research