Htr-9 stimulates ICAM-1 gene expression in human ASM cells. Htr-9 stimulates ICAM-1 gene expression in human ASM cells. A, ASM cells were stimulated with 30 μg/ml htr-9 for the indicated times. Five micrograms of total RNA was subjected to RT-PCR with the primers for ICAM-1 (lower band) and β-actin (upper band) as described in Materials and Methods. PCR products were separated on a 1% agarose gel and stained with ethidium bromide. B, scanning densitometry of gel. Data are representative of four separate experiments. Yassine Amrani et al. Mol Pharmacol 2000;58:237-245 The American Society for Pharmacology and Experimental Therapeutics