Kaviyanka Selvasandran, MS, Georges Makhoul, MD, MS, Prashant K

Slides:

Advertisements

Similar presentations

Human Cord Blood Stem Cells Enhance Neonatal Right Ventricular Function in an Ovine Model of Right Ventricular Training Ben Davies, MRCS(Eng), PhD, Ngaire.

Advertisements

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Uswa Shahzad, BHSc, Guangming Li, MD, Yaoguang Zhang, MD, Terrence M

Volume 6, Issue 6, Pages (June 2016)

Mammalian Cardiac Regeneration After Fetal Myocardial Infarction Requires Cardiac Progenitor Cell Recruitment Myron Allukian, MD, Junwang Xu, PhD, Michael.

Endothelial to Mesenchymal Transition Contributes to Endothelial Dysfunction in Pulmonary Arterial Hypertension Robert B. Good, Adrian J. Gilbane, Sarah.

Volume 15, Issue 6, Pages (June 2009)

Combretastatin A-4 prodrug inhibits growth of human non–small cell lung cancer in a murine xenotransplant model Arnd S Boehle, MD, Bence Sipos, MD, Ursula.

High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury

Transplantation of hypoxia-preconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure Oscar.

Mesenchymal Stem Cells Promote Formation of Colorectal Tumors in Mice

Cell therapy with autologous bone marrow mononuclear stem cells is associated with superior cardiac recovery compared with use of nonmodified mesenchymal.

Sirtuin1 Regulates the Stem Cell Therapeutic Effects on Regenerative Capability for Treating Severe Heart Failure in a Juvenile Animal Model Hideto Ozawa,

Neovasculogenic Therapy to Augment Perfusion and Preserve Viability in Ischemic Cardiomyopathy Pavan Atluri, MD, George P. Liao, MB, Corinna M. Panlilio,

How do cells talk to each other

Serum Biomarkers May Prognosticate Recurrence in Node-Negative, Non-Small Cell Lung Cancers Less Than 4 Centimeters Christopher W. Seder, MD, Andrew.

Faraz Kerendi, MD, Paul M. Kirshbom, MD, Michael E

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Aaron M. Abarbanell, MD, Arthur C. Coffey, MD, John W

Edaravone promotes activation of resident cardiac stem cells by transplanted mesenchymal stem cells in a rat myocardial infarction model Guang-Wei Zhang,

Duodenal Reflux Leads to Down Regulation of DNA Mismatch Repair Pathway in an Animal Model of Esophageal Cancer Pramod Bonde, MD, MS, Daqing Gao, PhD,

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage- Dominant Microenvironment in Lung Cancer Lunxu Liu, MD, PhD, Dongxia.

Pediatric End-Stage Failing Hearts Demonstrate Increased Cardiac Stem Cells Brody Wehman, MD, Sudhish Sharma, PhD, Rachana Mishra, PhD, Yin Guo, PhD,

Identifying Inhibitors of Epithelial-Mesenchymal Transition by Connectivity Map–Based Systems Approach Ajaya Kumar Reka, PhD, Rork Kuick, MS, Himabindu.

Engineering Patient-Specific Valves Using Stem Cells Generated From Skin Biopsy Specimens David L. Simpson, PhD, Brody Wehman, MD, Yekaterina Galat,

Intrathecal transplantation of bone marrow stromal cells attenuates blood-spinal cord barrier disruption induced by spinal cord ischemia-reperfusion injury.

Georges Makhoul, MD, MS, Ray C.J. Chiu, MD, PhD, Renzo Cecere, MD, MS

Pulmonary Fibroblasts Induce Epithelial Mesenchymal Transition and Some Characteristics of Stem Cells in Non-Small Cell Lung Cancer Yasushi Shintani,

Meena Nathan, MD, Ingeborg Friehs, MD, Yeong-Hoon Choi, MD, Douglas B

AKT2 Promotes Bone Marrow Cell-Mediated Aortic Protection in Mice

Shigetoshi Mieno, MD, Richard T

Changfa Guo, MD, Husnain Kh

Mesenchymal Stem Cells Promote Formation of Colorectal Tumors in Mice

Invited Commentary The Annals of Thoracic Surgery

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Sevoflurane Attenuates Ischemia-Reperfusion Injury in a Rat Lung Transplantation Model Akihiro Ohsumi, MD, Katherine Marseu, MD, Peter Slinger, MD, Karen.

Overexpression of transforming growth factor–β1 correlates with increased synthesis of nitric oxide synthase in varicose veins Theresa Jacob, PhD, Anil.

Aging impairs the angiogenic response to ischemic injury and the activity of implanted cells: Combined consequences for cell therapy in older recipients

Pingping Ren, MD, Lin Zhang, MS, Gaiping Xu, MD, Laura C

Transforming Growth Factor-β1 Mechanisms in Aortic Valve Calcification: Increased Alkaline Phosphatase and Related Events Jocelyn N. Clark-Greuel, PhD,

Human Cord Blood Stem Cells Enhance Neonatal Right Ventricular Function in an Ovine Model of Right Ventricular Training Ben Davies, MRCS(Eng), PhD, Ngaire.

Patrick D. Kohtz, MD, Alison L. Halpern, MD, Mohamed A

Vascular endothelial growth factor transgene expression in cell-transplanted hearts Terrence M. Yau, MD, MSc, Guangming Li, MD, Richard D Weisel, MD,

Osteopontin-Expressing Macrophages in Non-Small Cell Lung Cancer Predict Survival Yue Li, MD, Bing-sheng Sun, MD, PhD, Baoxiang Pei, MD, Chen-guang Li,

Michael G. Katz, MD, PhD, Anthony S. Fargnoli, PhD, Andrew P

Marrow Stromal Cells as Universal Donor Cells for Myocardial Regenerative Therapy: Their Unique Immune Tolerance Rony Atoui, MD, MS, Juan-Francisco Asenjo,

Expression of Bone Morphogenetic Proteins in Human Lung Carcinomas

Vascular Endothelial Growth Factor Prevents Endothelial-to-Mesenchymal Transition in Hypertrophy Ben M.-W. Illigens, MD, Alejandra Casar Berazaluce,

Therapeutic Potential of Human Umbilical Cord Derived Stem Cells in a Rat Myocardial Infarction Model Kai Hong Wu, MD, PhD, Bin Zhou, PhD, Cun Tao Yu,

Dexamethasone Pretreatment Provides Antiinflammatory and Myocardial Protection in Neonatal Arterial Switch Operation Ruth Heying, MD, PhD, Edith Wehage,

William T. Mahle, MD, Emilia Matthews, MS, Kirk R. Kanter, MD, Brian E

Percutaneous Tissue-Engineered Pulmonary Valved Stent Implantation

Lung cancer cell invasion and expression of intercellular adhesion molecule-1 (ICAM-1) are attenuated by secretory phospholipase A2 inhibition Jessica.

Induced Pluripotent Stem Cell Transplantation in the Treatment of Porcine Chronic Myocardial Ischemia Yifu Zhou, MD, Suna Wang, MD, Zuxi Yu, MD, PhD,

Promoting angiogenesis protects severely hypertrophied hearts from ischemic injury Ingeborg Friehs, MD, Adrian M Moran, MD, Christof Stamm, MD, Yeong-Hoon.

Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats Irshad Ali, PhD, Stephanie Gruenloh, BS, Ying Gao, MS,

Mammalian Cardiac Regeneration After Fetal Myocardial Infarction Requires Cardiac Progenitor Cell Recruitment Myron Allukian, MD, Junwang Xu, PhD, Michael.

Bret A. Mettler, MD, Virna L. Sales, MD, Chaz L

Volume 22, Issue 4, Pages (April 2014)

Impact of Normothermic Perfusion and Protein Supplementation on Human Endothelial Cell Function During Organ Preservation Thomas Puehler, MD, Otto Gleich,

Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis Masayuki Kubo, PhD, Tao-Sheng Li, MD, PhD, Takahiro.

Volume 21, Issue 2, Pages (February 2013)

Stem Cells in Thoracic Aortic Aneurysms and Dissections: Potential Contributors to Aortic Repair Ying H. Shen, MD, PhD, Xiaoqing Hu, MD, Sili Zou, MD,

Volume 6, Issue 6, Pages (June 2016)

Completely Thrombosed Tricuspid Pouch Mimicking a Cardiac Tumor

In Vivo Tracking of Mesechymal Stem Cells Using Fluorescent Nanoparticles in an Osteochondral Repair Model Jong Min Lee, Byung-Soo Kim, Haeshin Lee,

Chondrogenic progenitor cells promote vascular endothelial growth factor expression through stromal-derived factor-1 S. Wang, C. Zhou, H. Zheng, Z. Zhang,

Vascular Endothelial Growth Factor Receptor Upregulation in Response to Cell-Based Angiogenic Gene Therapy Terrence M. Yau, MD, MS, Guangming Li, MD,

Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production Michael R. Dashwood, PhD, Kay Savage, PhD, Audrey Dooley,

Presentation transcript:

A Tumor Necrosis Factor-α and Hypoxia-Induced Secretome Therapy for Myocardial Repair Kaviyanka Selvasandran, MS, Georges Makhoul, MD, MS, Prashant K. Jaiswal, PhD, Rishi Jurakhan, MS, Li Li, MS, Khalid Ridwan, MD, MS, Renzo Cecere, MD The Annals of Thoracic Surgery Volume 105, Issue 3, Pages 715-723 (March 2018) DOI: 10.1016/j.athoracsur.2017.09.005 Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Immunofluorescence reveals the expression of tumor necrosis factor receptors (TNFR)-1 and -2 (green) on the plasma membranes of (A and D) unconditioned and (B, C, E, F) preconditioned rat bone marrow mesenchymal stem cells. 4,’6-diamidino-2-phenylindole (DAPI) stains, visualized in blue, represent the nuclei of the cells (original magnification ×20). (CM = conditioned medium; TNF = tumor necrosis factor.) The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Immunofluorescence reveals the expression of tumor necrosis factor receptors (TNFR)-1 and -2 (green) on the plasma membranes of (A and D) unconditioned and (B, C, E, F) preconditioned rat bone marrow mesenchymal stem cells. 4,’6-diamidino-2-phenylindole (DAPI) stains, visualized in blue, represent the nuclei of the cells (original magnification ×20). (CM = conditioned medium; TNF = tumor necrosis factor.) The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (a) Western blot analysis was conducted on rat bone marrow mesenchymal stem cells (rBM-MSC) that were initially treated with combinations of H9c2 cardiomyoblast–conditioned media (CM), normoxia, hypoxia, and tumor necrosis factor (TNF)-α (50 ng/mL). Protein expression was normalized to β-actin. Increased expression of (c) angiopoietin-2 (Ang-2), (d) fibroblast growth factor (FGF)-2, (f) vascular endothelial growth factor (VEGF)-1, and (g) transforming growth factor (TGF)-β in TNF-α + H9c2-CM + hypoxia–treated cells was indicated compared with H9c2-CM + hypoxia–treated cells. (b) Ang-1 expression is decreased, whereas (e) FGF-7 expression remains unchanged. Fold differences were normalized to the untreated rBM-MSC group. The error bars on the graphs represent the SD. Statistical significance is at p < 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (a) Western blot analysis was conducted on rat bone marrow mesenchymal stem cells (rBM-MSC) that were initially treated with combinations of H9c2 cardiomyoblast–conditioned media (CM), normoxia, hypoxia, and tumor necrosis factor (TNF)-α (50 ng/mL). Protein expression was normalized to β-actin. Increased expression of (c) angiopoietin-2 (Ang-2), (d) fibroblast growth factor (FGF)-2, (f) vascular endothelial growth factor (VEGF)-1, and (g) transforming growth factor (TGF)-β in TNF-α + H9c2-CM + hypoxia–treated cells was indicated compared with H9c2-CM + hypoxia–treated cells. (b) Ang-1 expression is decreased, whereas (e) FGF-7 expression remains unchanged. Fold differences were normalized to the untreated rBM-MSC group. The error bars on the graphs represent the SD. Statistical significance is at p < 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Transwell cell migration assay shows that the tumor necrosis factor (TNF)-α + hypoxia–induced secretome enhances the migration of rat bone marrow mesenchymal stem cells (rBM-MSCs) compared with the control secretome (p < 0.05). The TNF-α + hypoxia–induced secretome cell count quantitatively lays closer to the stromal cell–derived factor-1 (SDF-1) positive control than the serum-free Dulbecco’s Modified Eagle’s Medium negative control. The bar graph depicts the cell count per mm2 postmigration in response to each factor. The error bars on the graphs represent the SD.*p < 0.05, ***p < 0.001. (CM = conditioned medium.) The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 A significant increase in the left ventricular fractional shortening (LVFS) of the rats treated with the tumor necrosis factor (TNF)-α and hypoxia–induced secretome is determined compared with the control secretome (p < 0.01). The error bars on the graphs represent the SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions

Supplemental Fig 1 The Annals of Thoracic Surgery 2018 105, 715-723DOI: (10.1016/j.athoracsur.2017.09.005) Copyright © 2018 The Society of Thoracic Surgeons Terms and Conditions