Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

Slides:

Advertisements

Similar presentations

Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine.

Advertisements

Volume 13, Issue 9, Pages (October 2011)

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

Volume 82, Issue 7, Pages (October 2012)

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Expression pattern differences between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation P. Bernstein, C. Sticht,

Chondro-protective effects of low intensity pulsed ultrasound

Chondrocytes extract from patients with osteoarthritis induces chondrogenesis in infrapatellar fat pad-derived stem cells E. López-Ruiz, M. Perán, J.

Bone morphogenetic protein (BMP)-2 enhances the expression of type II collagen and aggrecan in chondrocytes embedded in alginate beads Tatiana Gründer,

BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium- derived progenitor cells cultured in three-dimensional alginate hydrogel

Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation Y. Park, J. Hosomichi,

Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC- derived chondrocytes R.S. Rainbow, H. Kwon, A.T. Foote, R.C. Preda, D.L.

Glycoprotein Nonmelanoma Clone B Regulates the Crosstalk between Macrophages and Mesenchymal Stem Cells toward Wound Repair Bing Yu, Talib Alboslemy,

Regulation of mesenchymal stem cell chondrogenesis by glucose through protein kinase C/transforming growth factor signaling T.-L. Tsai, P.A. Manner,

Volume 10, Issue 1, Pages (January 2012)

MAPKs are essential upstream signaling pathways in proteolytic cartilage degradation – divergence in pathways leading to aggrecanase and MMP-mediated.

Stepwise preconditioning enhances mesenchymal stem cell-based cartilage regeneration through epigenetic modification S. Lin, W.Y.W. Lee, L. Xu, Y. Wang,

Volume 82, Issue 7, Pages (October 2012)

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

AG-041R, a novel indoline-2-one derivative, stimulates chondrogenesis in a bipotent chondroprogenitor cell line CL-1 Hidetomo Kitamura, D.V.M., Ph.D.,

Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao

TGFβ inhibition during expansion phase increases the chondrogenic re-differentiation capacity of human articular chondrocytes R. Narcisi, L. Signorile,

Volume 182, Issue 1, Pages (July 2009)

Volume 9, Issue 4, Pages (April 2004)

Subtractive gene expression profiling of articular cartilage and mesenchymal stem cells: serpins as cartilage-relevant differentiation markers S. Boeuf,

Functional consequences of glucose and oxygen deprivation on engineered mesenchymal stem cell-based cartilage constructs M.J. Farrell, J.I. Shin, L.J.

Sequential exposure to fibroblast growth factors (FGF) 2, 9 and 18 enhances hMSC chondrogenic differentiation D. Correa, R.A. Somoza, P. Lin, S. Greenberg,

Gene expression and cell differentiation in matrix-associated chondrocyte transplantation grafts: a comparative study C. Albrecht, B. Tichy, S. Nürnberger,

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

Identification, quantification and isolation of mesenchymal progenitor cells from osteoarthritic synovium by fluorescence automated cell sorting S Fickert,

Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture Dr R.L. Mauck, Ph.D., X. Yuan, Dr.

Isolation of multipotent mesenchymal stem cells from umbilical cord blood by Oscar K. Lee, Tom K. Kuo, Wei-Ming Chen, Kuan-Der Lee, Shie-Liang Hsieh, and.

S. Varghese, Ph. D. , P. Theprungsirikul, B. S. , S. Sahani, B. S. , N

Toward scaffold-based meniscus repair: effect of human serum, hyaluronic acid and TGF-ß3 on cell recruitment and re-differentiation U. Freymann, M. Endres,

Glucosamine promotes chondrogenic phenotype in both chondrocytes and mesenchymal stem cells and inhibits MMP-13 expression and matrix degradation A.

Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation D.C. Lacey, P.J. Simmons,

Human synovial fluid derived mesenchymal stem cells expanded under low oxygen conditions and in a serum-free environment exhibit enhanced lineage-specific.

Synthetic triterpenoids, CDDO-Imidazolide and CDDO-Ethyl amide, induce chondrogenesis N. Suh, S. Paul, H.J. Lee, T. Yoon, N. Shah, A.I. Son, A.H. Reddi,

A. H. Huang, B. S. , M. Yeger-McKeever, M. D. , A. Stein, R. L

Human Dermis Harbors Distinct Mesenchymal Stromal Cell Subsets

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Y. Hagiwara, A. Ando, Y. Onoda, T. Takemura, T. Minowa, N. Hanagata, M

Involvement of Gas7 along the ERK1/2 MAP kinase and SOX9 pathway in chondrogenesis of human marrow-derived mesenchymal stem cells Y. Chang, M.D., S.W.N.

The extent of degeneration of cruciate ligament is associated with chondrogenic differentiation in patients with osteoarthritis of the knee K. Kumagai,

E. Farrell, P. Wielopolski, P. Pavljasevic, N. Kops, H. Weinans, M. R

Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage Y. Yu, H. Zheng, J.A. Buckwalter, J.A. Martin

Osteoarthritis and Cartilage

Low calcium levels in serum-free media maintain chondrocyte phenotype in monolayer culture and reduce chondrocyte aggregation in suspension culture A.

A predominantly articular cartilage-associated gene, SCRG1, is induced by glucocorticoid and stimulates chondrogenesis in vitro Kensuke Ochi, M.D., Ph.D.,

Immature murine articular chondrocytes in primary culture: a new tool for investigating cartilage Colette Salvat, B.Sc., Audrey Pigenet, Lydie Humbert,

T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M

M. A. Cleary, R. Narcisi, K. Focke, R. van der Linden, P. A. J

Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications

Pharmaceutical nanocarrier association with chondrocytes and cartilage explants: influence of surface modification and extracellular matrix depletion

Hypoxia differentially regulates human nucleus pulposus and annulus fibrosus cell extracellular matrix production in 3D scaffolds G. Feng, L. Li, H.

Spatial regulation of human mesenchymal stem cell differentiation in engineered osteochondral constructs: effects of pre-differentiation, soluble factors.

Growth characterization of neo porcine cartilage pellets and their use in an interactive culture model Carsten Lübke, Ph.D., Jochen Ringe, M.Sc., Veit.

Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins M. Shimaya, T. Muneta, S. Ichinose, K. Tsuji,

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

Volume 4, Issue 3, Pages (March 2015)

Tissue engineering with meniscus cells derived from surgical debris

Membrane culture and reduced oxygen tension enhances cartilage matrix formation from equine cord blood mesenchymal stromal cells in vitro C. Co, M.K.

Kim L. Kroeze, Wouter J. Jurgens, Behrouz Z. Doulabi, Florine J

Enhanced phagocytic capacity endows chondrogenic progenitor cells with a novel scavenger function within injured cartilage C. Zhou, H. Zheng, J.A. Buckwalter,

Chondro-protective effects of low intensity pulsed ultrasound

Volume 8, Issue 6, Pages (June 2017)

Inhibition of miR-449a Promotes Cartilage Regeneration and Prevents Progression of Osteoarthritis in In Vivo Rat Models Dawoon Baek, Kyoung-Mi Lee, Ki.

Effect of expansion medium on ex vivo gene transfer and chondrogenesis in type II collagen–glycosaminoglycan scaffolds in vitro R.M. Capito, Ph.D., M.

Presentation transcript:

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K. Aicher Osteoarthritis and Cartilage Volume 17, Issue 10, Pages 1368-1376 (October 2009) DOI: 10.1016/j.joca.2009.04.023 Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Proliferation of MSC. Displayed are mean values and SD of numbers of MSC from three donors expanded over four passages either in FCS-free FFPP medium (red line) or in FCS-containing MSCGM medium (black line). MSC expanded in FCS-free FFPP medium are characterized by significantly higher proliferation rates than MSC expanded in FCS-containing MSCGM medium. Osteoarthritis and Cartilage 2009 17, 1368-1376DOI: (10.1016/j.joca.2009.04.023) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Immunophenotype. Flow cytometric analysis of cell surface antigens of MSC expanded in FCS-free FFPP medium revealed expression of CD73, CD90, CD105 and a lack of expression of CD11b, CD34 and CD45. The histograms display the fluorescence profiles of MSC stained with the mAB (red lines) and the unstained controls (green lines). Osteoarthritis and Cartilage 2009 17, 1368-1376DOI: (10.1016/j.joca.2009.04.023) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Differentiation capacity of MSC. MSC were expanded in FFPP or MSCGM medium without induction of differentiation. On day 28 of expansion differentiation was induced. A similar differentiation into adipogenic (a, b), osteogenic (c, d) and chondrogenic (e, f) lineages was observed from MSC expanded in FCS-free FFPP medium (a, c, e) and FCS-containing MSCGM medium (b, d, f): Oil Red O staining (a, b) shows adipocytes filled with lipid vesicles. Von Kossa staining (c, d) indicates calcified areas generated by osteoblasts. Alcian blue staining (e, f) illustrates proteoglycan distribution of prepared 7μm cryosections in micromasses. Scale bars=200μm. Osteoarthritis and Cartilage 2009 17, 1368-1376DOI: (10.1016/j.joca.2009.04.023) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Characterization of chondrogenically differentiated micromass pellets under influence of IL-1β, under hypoxia in presence of IL-1β, and under hypoxia. Morphology of generated micromass pellets. Pictures of micromasses were taken in cell culture wells after 4 weeks of chondrogenic differentiation (a–d; scale bars=200μm). The expression of proteoglycan in micromasses was detected in frozen sections by Alcian blue staining (e–h; scale bars=200μm). Note that IL-1β reduced Alcian blue staining intensity to a pale blue, indicating low aggrecan expression (f). The expression of type I (i–l) and type II (m–p) collagens was investigated in frozen sections by immunohistochemistry. Nuclei were counterstained with 4′,6-Diamidino-2-phenylindole (DAPI). The figure presents the overlay of micrographs utilizing the PE and DAPI channels (i–p) (size bars=200μm). Osteoarthritis and Cartilage 2009 17, 1368-1376DOI: (10.1016/j.joca.2009.04.023) Copyright © 2009 Osteoarthritis Research Society International Terms and Conditions