Volume 108, Issue 1, Pages 233-239 (July 1995) Expression of Cell Adhesion Molecules in the Lungs of Patients With Idiopathic Pulmonary Fibrosis  Akihiro Nakao, MD, Yoshinori Hasegawa, MD, FCCP, Yoshiki Tsuchiya, MD, Kaoru Shimokata, MD, FCCP  CHEST  Volume 108, Issue 1, Pages 233-239 (July 1995) DOI: 10.1378/chest.108.1.233 Copyright © 1995 The American College of Chest Physicians Terms and Conditions

Figure 1 Normal lung tissues immunohistochemically stained with MoAb for (top, A) ICAM-1, (center, B) E-selectin, and (bottom, C) VCAM-1. Top, A, Small vessel endothelial cells (arrow) are weakly and alveolar epithelial cells (arrowheads) are moderately stained with ICAM-1. Center, B, and bottom, C, E-selectin and VCAM-1 are not stained (original magnification ×345). Bar=50 µm. CHEST 1995 108, 233-239DOI: (10.1378/chest.108.1.233) Copyright © 1995 The American College of Chest Physicians Terms and Conditions

Figure 2 Serial sections of a region of interstitial pneumonitis in a patient with IPF immunohistochemically stained with MoAb for (top, A) ICAM-1, (center, B) E-selectin, and (bottom, C) VCAM-1 (case 1). Top, A, Small vessels (arrows) and alveolar capillaries (small arrowheads) are moderately stained with ICAM-1. Regenerating type 2 alveolar epithelial cells (large arrowheads) are strongly stained, and clustered alveolar macrophages (open triangle) are also stained. Center, B, E-selectin is not or only faintly stained on small vessels (arrow). Bottom, C, VCAM-1 is negative. Open arrowheads indicate deposition of dust particles (original magnification ×173). Bar=100 μm. CHEST 1995 108, 233-239DOI: (10.1378/chest.108.1.233) Copyright © 1995 The American College of Chest Physicians Terms and Conditions

Figure 3 Serial sections of honeycomb region of a patient with idiopathic pulmonary fibrosis immunohistochemically stained with MoAb for (top, A) ICAM-1, (center, B) E-selectin, and (bottom, C) VCAM-1 (case 1). Top, A, Small vessels (arrows) are moderately stained with ICAM-1. Metaplastic epithelial cells (arrowheads) and alveolar macrophage (open triangle) are also stained. Center, B, Small vessels (arrows) are moderately stained with E-selectin. Bottom, C VCAM-1 is not stained. Open arrowheads indicate deposition of dust particles (original magnification ×138). Bar=100 μm. CHEST 1995 108, 233-239DOI: (10.1378/chest.108.1.233) Copyright © 1995 The American College of Chest Physicians Terms and Conditions

Figure 4 Immunoelectron microscopy for E-selectin in honeycomb region of a patient with idiopathic pulmonary fibrosis (case 1). Immunoreactivity (arrowheads) is observed along the luminal plasma membrane of endothelial cells of a venule. E=endothelial cell; N=neutrophil (original magnification ×5,800). Bar=3 μm. CHEST 1995 108, 233-239DOI: (10.1378/chest.108.1.233) Copyright © 1995 The American College of Chest Physicians Terms and Conditions