CLINICAL PATHOLOGY: C-Reactive Protein GENERAL OBJECTIVE: After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem SPECIFIC OBJECTIVE: At the end of lab activity of C-RP, the student will be able to interprete: - the normal and abnormal C-RP

C-Reactive Protein C-RP is a serum constituent originally define by its ability to precipitate Pneumococcus C polysaccharide C-RP is an acute phase reactant: A protein synthesized by the liver as a non-specific response to the inflammation

C-Reactive Protein C-RP concentration increased in the serum of individu as a response to varies inflammatory condition and tissue necrosis before specific antibody production starts and the ESR is increased. After successful therapy, CRP level fall before the ESR decreases

C-Reactive Protein Specimen: serum Reference range: 1. < 6 mg/L (aglutination method), semiquantitative 2. Immunoturbidimetric (quantitative) < 5 mg/L

C-Reactive Protein Method: Latex agglutination Specimen: Serum Principle: Monoclonal anti C-RP will binds and agglutinate the CRP in the specimen

PROCEDURE Allow each component of reagents and specimen to reach room temperature Gently shake the reagent Place a drop of undiluted specimen to the slide Add one drop of the latex reagent next to the drops of specimen Spread the reagent and specimen over theentire area of test circle Gently tilt the test slide backwards and forwards for two minutes. Positive and negative controls should be included

INTERPRETATION Presence of agglutination indicates a level of C-RP in the specimen equal or > 6mg/L The lack of agglutination indicates a C-RP level < 6 mg/L