Reduced turn-around time for Mycobacterium tuberculosis drug susceptibility testing with a proportional agar microplate assay V.A.T. Nguyen, H.Q. Nguyen,

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Presentation transcript:

Reduced turn-around time for Mycobacterium tuberculosis drug susceptibility testing with a proportional agar microplate assay V.A.T. Nguyen, H.Q. Nguyen, T.T. Vu, N.A.T. Nguyen, C.M. Duong, T.H.T. Tran, H.V. Nguyen, D.A. Dang, A.-L. Bañuls Clinical Microbiology and Infection Volume 21, Issue 12, Pages 1084-1092 (December 2015) DOI: 10.1016/j.cmi.2015.08.024 Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Reading of results in a 12-well microplate drug susceptibility testing assay. VNM7: Mycobacterium tuberculosis isolate resistant to isoniazid (INH) (left half of the 12-well plate); VNM33: M. tuberculosis isolate resistant to INH, rifampicin (RMP), and streptomycin (SM) (right half of the 12-well plate). Photograph taken at day 11 of culture. Clinical Microbiology and Infection 2015 21, 1084-1092DOI: (10.1016/j.cmi.2015.08.024) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Mycpbacterium tuberculosis growth on a control well of the 12-well microplate from day 1 to day 12 (control well: 105 CFU/mL bacterial solution and no drug), monitored with a low-magnification (10×) microscope. Clinical Microbiology and Infection 2015 21, 1084-1092DOI: (10.1016/j.cmi.2015.08.024) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions