FlexarTM: Advances in HPLC Sample Throughput

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Presentation transcript:

FlexarTM: Advances in HPLC Sample Throughput Kim, Wang-Yu PhD PerkinElmer Korea © 2009 Perkin Elmer

Flexar. More choices in LC analysis. 18,000 PSI …the most choices in pump pressures

Flexar Features …designed with form and function in mind Tubing management for streamlined chromatography Bottle tray with integrated degassing and SW comm link Elegant, ergonomic user interface with streamlined, consistent look & footprint Inter-component drain management EACH FLEXAR LC SYSTEM IS BUILT ON A PERFORMANCE PUMP AND A SOLVENT MANAGEMENT PLATFORM A STREAMLINED DESIGN OFFERS COMPACT ELEGANCE, COMPATABILITY FOR SEVERAL INTEGRATED SYSTEMS & LOW VOLUME PLUMBING TO ELIMINATE EXTRA-COLUMN DISPERSION THAT CAN ADVERSELY AFFECT YOUR CHROMATOGRAPHY. EACH FLEXAR SYSTEM IS ASSEMBLED FROM A VARIETY OF COMPONENTS TO MEET YOUR DEMANDING NEEDS FOR PERFORMANCE & FLEXABILITY ALL WITH CHOICES IN INSTRUMENT CONTROL SOFTWARE Built on rugged PerkinElmer LC technology, recognized for reliability Controlled by both Chromera and TotalChrom …designed with form and function in mind

Flexar Tubing Management System LC Tube Management HPLC tubing is conveniently and efficiently managed using built-in tubing clips, located in all four corners, for flexibility and efficiency in configuring many different systems. …streamlined design for flexibility and low dwell volume

Flexar Drainage System Hidden inter-component drainage system Plug-and-play drainage system automatically interconnects with each component added to the stack. Works behind the scenes; protecting the instrument and the lab space from leaks. …works behind the scenes

Flexar Solvent Manager Three versions available Without degassing 3-channel degassing 5-channel degassing Can be combined with any Flexar pumps Solvent delivery tubes conveniently managed through rear manifold. Can hold up to five 1–liter solvent bottles Built-in communications link and vacuum degasser Stackable design, with Flexar tube management and built-in inter-component drain system Removable tray to contain breach of complete bottle …more than just a bottle tray

Flexar Autosampler …absolutely accessible for easy loading and service Open Access …absolutely accessible for easy loading and service

Flexar HPLC Pumps PerkinElmer HPLC pumps clock in at >4400 hrs. MTBF – more than two years of continuous operation without failure. ... Our pumps are recognized throughout the industry as the most rugged and reliable. Easy to learn, easy to own – an elegant, ergonomic design optimizes tubing flow paths, manages drainage and makes all Flexar components compatible in an intuitive package, which minimizes dead volume and maximizes operator safety. Reduced business risk – with the best reliability in the industry PerkinElmer’s LC pumps assure your job gets done, today and tomorrow. Low cost of ownership – PerkinElmer’s pump piston technology keeps you up and running day in and day out. Maintaining strict alignment of the precision pump piston greatly reduces seal wear and greatly extends the time between maintenance. Future proofing – upgrade 6000 psi pumps from isocratic to binary or to quaternary pumping as your needs grow. Reach for more choices in LC analysis.

And now Flexar UHPLC takes this to the next level Flexar Pumps And now Flexar UHPLC takes this to the next level No compromises – the widest choice of operating modes, models and configurations assures that you can configure the best solution precisely tailored to your application needs. Improved productivity – our Flexar FX-10 and FX-15 UHPLC pumps improve throughput by as much as a factor of 10x, getting more samples done, faster. Reduced solvent consumption – operation with sub-2µm column technology, our UHPLC pumps reduce solvent consumption by as much as 93% – greatly minimizing your waste disposal needs and reducing your operating costs. Improved separation efficiency – our Flexar UHPLC pumps can deliver separation efficiency enhancements of 5-10x, allowing you to separate compounds better than you ever have before. Reach for more choices in LC analysis.

Flexar UHPLC Systems… …You don’t have to pay more to achieve so much. FX-10 UHPLC System FX-15 UHPLC System FX-10: High Value UHPLC Micro Binary 10,000 psi pump package FX-15: Ultimate in UHPLC Dual reciprocating 18,000 psi pump High efficiency FX UHPLC autosampler Column oven for retention time reproducibility and viscosity reduction High speed FX UV/VIS detector (100 pt/sec) with high efficiency 2.4µL flow cell …You don’t have to pay more to achieve so much.

Flexar FX-15 UHPLC Pump …the ultimate in UHPLC 15,0000 psi operation for the most demanding UHPLC applications with up to 10x productivity improvement – up to 5mL/min at 18,000 psi! Green productivity –mobile phase solvent consumption reduced by 10-15x Dual reciprocating 15,000 psi pump Smoother more precise flow for retention time repeatability Optical Sensor synchronizes injection with piston position Maximum retention time repeatability for UHPLC High pressure Ti-tip check valves and pulse dampeners Rated to >15,000 psi operation for highest throughput UHPLC Integrated piston wash function Keeps precision pumps clean even with buffers …the ultimate in UHPLC

Flexar FX-15 Pump for Ultimate UHPLC Exclusive Ultra High Pressure Pulse Dampeners Rated to >18,000 p.s.i. Significantly reduces pulsation. Innovative Check “T” Prevents channel-to-channel cross talk. No back flow to affect composition. Improved retention time repeatability.

FX-15 UHPLC Pump Piston Drive Automatic Piston Wash Greatly Increases Seal Life (particularly with buffers) No Auxiliary Pump Required (self flush) Dedicated recirculation flush solvent 15 µL displacement Self-centering piston saddle Captive spring for fast head removal

Flexar: FX-15 Pump [Retention time repeatability] Methyl benzoate (MB) and Ethyl benzoate (EB) Pressure Column Flow (MB) RT %RSD (EB) RT %RSD 1,268 psi 5µmx4.6x15 1.5 mL/min 0.06% 0.08% 8,056 psi 3µmx2.1x250 0.7 mL/min 0.24% 0.27% 11,500 psi 1.5µmx2.0x100 0.7mL/min 0.28% 0.14% …retention time reliability over a broad range

Flexar: Autosampler …speed, precision, accuracy & thermal stability Flexar Autosampler: Up to 6,500 psi (450 bar) FX UHPLC Autosampler: Up to 18,000 psi (1035 bar) In partial fill mode, can load/inject sample in 8 sec 1 – 2500µL injection volume capacity Supports full loop, partial fill and µL-pickup injection modes µL-pickup mode = zero sample waste Supports Peltier cooling/heating mode option 4 ºC (± 2 ºC) achievable @ ambient temperature of 25 ºC ! All Trays temperature controlled …speed, precision, accuracy & thermal stability

Flexar Autosamplers: World-class Performance Excellent Injection Repeatability Above 5µL injections, %RSDs of 0.5% For 5µL injections, in µL-Pickup mode, %RSDs are also typically 0.5% Area %RSDs fixed loop mode of 0.3% For 2µL injections, in µL-Pickup mode, (10µL loop) area %RSDs are typically 0.5% Anthracene area precision: 0.24% RSD 5µL injection n=30 (1) 300 ppm caffeine (2) Blank injection (3) 30µg/L caffeine Very low carryover: Pressure assist air purge after each run …what carry over?

Flexar LC Column Oven Three versions Heat only Peltier (heat and cool) Peltier with column selection/switching (TotalChrom only – Chromera coming) Built-in leak alarm Integrated solvent pre-heater/chiller minimizes temperature gradients Better column performance More repeatable retention times Temperature range of 30ºC to 90ºC (5ºC to 90ºC for Peltier), controlled to within 0.2ºC throughout entire temperature range Stackable design, with Flexar tube management and built-in inter-component drain system Large, easily accessible column compartment holds even 30-cm column format …Precise temperature control for improved retention time stability.

Flexar UV/VIS Detectors… FX UV/VIS UHPLC Detector World-class UV/VIS detection Dual beam optical design with choice of tungsten or deuterium light sources with wavelength range of 190-700 nm Up to 50 pts/sec 10 µL flow cell standard – compatible with a wide range of optional flow cells Fast, sharp UHPLC performance High efficiency 2.4 µL flow cell for optimum UHPLC peak resolution 100 pts/sec detection to capture even the fastest UHPLC peaks UV/VIS LC Detector …sensitive, selective, speed, dynamic range

IRIS Spectral Processing Software Flexar PDA Detectors… FX PDA UHPLC Detector Excellent performance, HPLC or UHPLC Improved noise specifications (<0.9 x 105), while maintaining excellent signal-to-noise, wide linear range and low drift Best-in-class spectral integrity delivers improved peak identification and purity FX version: High efficiency 2.4uL flow cell with detector electronics optimized for flow cell IRIS Spectral Processing Software PDA LC Detector ...Deeper insights with more powerful methods development.

Flexar Refractive Index and Fluorescence Detectors… Refractive Index Detector Combine sensitivity and specificity Best sensitivity in the market Now under Chromera control and can conveniently be combined with UV/VIS detection (PAH) Rugged general-purpose detection Highly stable and sensitive LC and GPC detector for compounds that do not have high UV absorbance such as polymers, sugars, organic acids and triglycerides Internal temperature of flow cell for baseline stability Autozero and autopurge of reference cell make it easy to use Fluorescence Detector …now fully controlled under Chromera!

Flexar. Reach for more choices in LC analysis. FX-15 15,000 psi Pumps FX-10 pump package FX-15 Autosamplers FX-10 10,000 psi Manual injection systems Flexar 6,000 psi PDA UV/VIS FL RI WIDEST CHOICE IN OPERATING PRESSURES: 6k psi → 10k psi → 15k psi NEW HIGH-END 15K psi UHPLC SYSTEM BEST PRICE-TO-PERFORMANCE RATIO BUILT ON FIELD PROVEN RELIABILITY AND BACKED BY THE INDUSTRIES LARGEST SERVICE & SUPPORT ORGANIZATION Ovens

Reach for more choices in LC analysis. Flexar: The most complete line of flexible LC solutions for all your separation needs. Choice: The most choice in pumping configurations, operating pressures, detectors, accessories and CDS (Chromatography Data System) software platforms. Easy to use, easy to own: New streamlined ergonomic package with optimized tubing management for enhanced performance and easier operation. Reliability: Built on PerkinElmer’s rugged LC platform, long recognized for its reliable operation. Support: Supported by the industry’s largest global applications and service support network. Experience and commitment: Inspired by 40 years of innovation in LC technology development and commercialization. Reach for more choices in LC analysis.

Thank you.