Supplementary Figure 1 A B wild type ΔacsS

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Supplementary Figure 1 A B wild type ΔacsS 500 bp acsS upR downF upF downR VVMO6_00191 wild type ΔacsS SM (bp) wild type ΔacsS 6,000 1,000 Supplementary Figure 1 Construction of acsS mutant V. vulnificus. A - Construction of V. vulnificus mutant defective in acsS by using two sets of primers (indicated by horizontal arrows with the primer names listed in Supplementary Table 2) to delete VVMO6_00191. A bar represents the length of DNA equivalent to 500 bp; B - Deletion of the corresponding gene was examined by PCR using a pair of primers, acsSupF and acsSdownR. SM indicates DNA size markers.