miRNAs regulated by c‐Myc in murine ES cells.

Slides:

Advertisements

Similar presentations

Prostaglandin E2 (PGE2) induces no significant alteration in circadian locomotor activity. Prostaglandin E2 (PGE2) induces no significant alteration in.

Advertisements

Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted ES cells on LIF withdrawal. Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted.

Knockdown of GW182 and TNRC6B impaired miRNA stability.

Reduced astrocyte proliferation results in increased immune cell infiltration into the injured GM Reduced astrocyte proliferation results in increased.

Nur77 interacts with COUP‐TF in yeast.

Increased proliferation and delayed differentiation of adult primary myoblasts derived from MyoDR99,102 mice. Increased proliferation and delayed differentiation.

JNK is activated by Wnt5a.

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

Downregulation of Prdm16 mRNA is a specific antileukemic mechanism during HOXB4-mediated HSC expansion in vivo by Hui Yu, Geoffrey Neale, Hui Zhang, Han.

Matrix pH elevations propagate along connected, but not fused, mitochondria. Matrix pH elevations propagate along connected, but not fused, mitochondria.

MiR‐10b* down‐regulates the expression of BUB1, PLK1 and CCNA2 proteins in breast cancer cell lines.A.SPIN‐ordered expression matrix of miR‐10b* and its.

Morphological analyses of peripheral blood and bone marrow cells isolated from leukemic mice. Morphological analyses of peripheral blood and bone marrow.

NS3 inhibits endogenous miRNA action.

RNAi Delivers Insights into Liver Cancer

Differential regulation of SRF and NFAT by Ca2+.

The mRNA stem cell signature.

Comparative analysis of RNA and protein profiles.

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

Volume 11, Issue 12, Pages (June 2015)

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

Validation cell‐type‐enriched culturing methods, metabolome dynamics, and mitochondria abundance and bioenergetics in differentiating organoids Validation.

HS induces sustained H3K4me3 and H3K4me2 methylation at HSP22

Identification of genes associated with a gain of prion susceptibility Increased susceptibilities of revertant cell clones R2, R5 and R7 after retinoic.

Reactive oxygen species inhibit miR‐199a and miR‐125b expression levels in vitro and in vivo. Reactive oxygen species inhibit miR‐199a and miR‐125b expression.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Functional miR‐33 responsive elements in the 3′UTR of ATP8B1 and ABCB11.A,B.Conserved sequences in the 3′UTR of ATP8B1 and ABCB11 are partially complementary.

Activin reprograms macrophages toward a TAM‐like phenotype

(A) Schematic representation of the conditional Lats1 locus.

A B C E F D IC50 = 567 nM IC50 = 83 nM HAP1 WT HAP1 TOPK

Cocaine exposure downregulates miR-125b expression.

WDR11 cooperates with Hh pathway to regulate gene expression

Usp5 sensitivity of S65 ubiquitin mutant chains Structure of ubiquitin in complex with Usp5 (PDB ID: 3IHP). Usp5 sensitivity of S65 ubiquitin mutant chains.

Mapping of small RNAs to LINE‐1 sequence (related to Figs 3 and 4)‏

Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation.

Effect of Zc3h10 overexpression in myoblasts and myotubes

System approaches to analyze the effects of Zc3h10 knockdown

Myc increases the instability of lac operator repeats.

Kenichi Miharada, Valgardur Sigurdsson, Stefan Karlsson Cell Reports

Downregulation of human SSU72 results in telomere elongation and fragility Downregulation of human SSU72 results in telomere elongation and fragility Telomere.

Volume 63, Issue 4, Pages (August 2016)

USP9X and XIAP promote lymphoma growth and resistance to spindle poisons in vivo USP9X and XIAP promote lymphoma growth and resistance to spindle poisons.

HBL1 Is a Human Long Noncoding RNA that Modulates Cardiomyocyte Development from Pluripotent Stem Cells by Counteracting MIR1 Juli Liu, Yang Li, Bo Lin,

SGK3 Mediates INPP4B-Dependent PI3K Signaling in Breast Cancer

Volume 21, Issue 1, Pages (July 2004)

Myeloma cell–derived Runx2 promotes myeloma progression in bone

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

HBL1 Is a Human Long Noncoding RNA that Modulates Cardiomyocyte Development from Pluripotent Stem Cells by Counteracting MIR1 Juli Liu, Yang Li, Bo Lin,

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

HDAC1 positively regulates the abundance of miRNAs.

Hccs downregulation induces apoptosome‐independent caspase‐9 activation in mitochondria.A–C.TUNEL assays on st30 embryos co‐injected with hccs‐MO and Apaf1‐MO.

Categorizing cell type‐specific auxin responses.

Volume 27, Issue 5, Pages (November 2007)

Delivery of therapeutics to patients.

Trop‐2 can bind to IGF‐1 and interfere with IGF‐1R signalling.

In vivo efficacy of Olaparib in PTEN deficient xenografts.

Expression profiling along the intestinal crypt axis.

Change in mRNA expression of Glasso network genes in differentiated PC12 cells following Caprin2 knockdown or Caprin2 overexpression. Change in mRNA expression.

CD44 binding surface of the Tiam2 PHCCEx domain.

Scheme of FGF‐dependent regulation of TGFβ signaling in smooth muscle cells and endothelial cells Scheme of FGF‐dependent regulation of TGFβ signaling.

Existence of a nuclear NFATc1–STAT3 complex in pancreatic cancer.

Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling. Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling.

ADAM8 knockdown profoundly decreases orthotopic tumor formation, CTC spread and metastasis A–DStable ADAM8 KD (shA8) clones were characterized in vitro.

KLF4 regulates the EndMT switch in CCM1 KO ECs

Identification of ARRDC3 as a new gene required for β2AR degradation and ubiquitination. Identification of ARRDC3 as a new gene required for β2AR degradation.

Intestinal differentiation is coupled with global rewiring of gene expression Intestinal differentiation is coupled with global rewiring of gene expression.

CAR T‐cell generation in human PBMC‐transplanted mice

Volume 17, Issue 4, Pages (October 2015)

IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.

LATS2 augments oxidative phosphorylation.

Loss of polarity gene Dlg1 leads to an expansion of C'-1 stage cells during pre-B cell differentiation. Loss of polarity gene Dlg1 leads to an expansion.

Presentation transcript:

miRNAs regulated by c‐Myc in murine ES cells. miRNAs regulated by c‐Myc in murine ES cells. (A) Scheme showing the experimental design for identification of miRNAs regulated by c‐Myc in ES cells. c‐Myc level was increased by lentiviral‐delivered overexpression of c‐Myc in ES cells (see Supplementary Figure S1). These ES cells, their differentiated haematopoietic stem/progenitor cell derivatives (HSP Myc+) and tumours after injection of HSP c‐Myc+ cells into irradiated mice, were subjected to miRNA profiling. (B) Heatmap of miRNAs displaying significant regulation by c‐Myc in ES cells and ES cell‐derived differentiated cells or tumours. HSP: haematopoietic stem/progenitor cells derived by differentiation of ES cells; Tumours1–4: mixed T‐ and B‐cell tumours derived by transplantation of Myc+ HSPs into irradiated mice. Note that results are shown for two HSP, and four tumour cell isolates independently derived from the same ES cell line. Bottom row shows miRNAs levels after c‐Myc knockdown in WT ES cells. Inset: Fold change in miRNA copy number represents the ratio of miRNA level in ES cell in which c‐Myc has been introduced (Myc+), or knocked down by c‐myc shRNA (ES Myc KD), relative to lentiviral vector (see Supplementary Figure S1 for quantitation). Grey boxes represent no detectable signal. Chin‐Hsing Lin et al. EMBO J. 2009;28:3157-3170 © as stated in the article, figure or figure legend