Vascular Trauma Induces Rapid but Transient Mobilization of VEGFR2+AC133+ Endothelial Precursor Cells by Muhammad Gill, Sergio Dias, Koichi Hattori, Mary.

Slides:

Advertisements

Similar presentations

Jianfei Xie Introduction Hemophilia A is characterized by inability to clot blood because of FVIII gene mutations and deficiency of this coagulation.

Advertisements

Date of download: 7/9/2016 Copyright © The American College of Cardiology. All rights reserved. From: CD40 Ligand+ Microparticles From Human Atherosclerotic.

Oxidized Low-Density Lipoprotein Augments and 3- Hydroxy-3-Methylglutaryl Coenzyme A Reductase Inhibitors Limit CD40 and CD40L Expression in Human Vascular.

Westerweel PE et al. Eur Heart J 2008;29:

Interleukin 12 Induces T-Cell Recruitment Into the Atherosclerotic Plaque by Xiaoyu Zhang, Alexander Niessner, Takako Nakajima, Wei Ma-Krupa, Stephen L.

Acute Depletion of Endothelial β3-Integrin Transiently Inhibits Tumor Growth and Angiogenesis in MiceNovelty and Significance by Veronica Steri, Tim S.

by Hideto Sano, Kohei Hosokawa, Hiroyasu Kidoya, and Nobuyuki Takakura

Arterioscler Thromb Vasc Biol

by Johannes B. K. Schwarz, Nicolas Langwieser, Nicole N

Vascular Endothelial–Cadherin Tyrosine Phosphorylation in Angiogenic and Quiescent Adult Tissues by Nathalie Lambeng, Yann Wallez, Christine Rampon, Francine.

Vascular Progenitor Cells in Diabetes Mellitus

Mouse Carotid Artery Ligation Induces Platelet-Leukocyte–Dependent Luminal Fibrin, Required for Neointima Development by Tomihisa Kawasaki, Mieke Dewerchin,

Potassium Channels and Proliferation of Vascular Smooth Muscle Cells

TRAF5 Deficiency Accelerates Atherogenesis in Mice by Increasing Inflammatory Cell Recruitment and Foam Cell FormationNovelty and Significance by Anna.

by Matilde Murga, Oscar Fernandez-Capetillo, and Giovanna Tosato

Monocytes form a vascular barrier and participate in vessel repair after brain injury by John Glod, David Kobiler, Martha Noel, Rajeth Koneru, Shoshana.

In vivo administration of vascular endothelial growth factor (VEGF) and its antagonist, soluble neuropilin-1, predicts a role of VEGF in the progression.

by Benjamin J. Frisch, John M. Ashton, Lianping Xing, Michael W

Volume 130, Issue 2, Pages (February 2006)

Von Willebrand factor fibers promote cancer-associated platelet aggregation in malignant melanoma of mice and humans by Alexander T. Bauer, Jan Suckau,

The κ opioid system regulates endothelial cell differentiation and pathfinding in vascular development by Kohei Yamamizu, Sadayoshi Furuta, Shiori Katayama,

Direct Comparison of Umbilical Cord Blood versus Bone Marrow–Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular.

Vascular leukocytes contribute to tumor vascularization

by Thomas Rasmussen, Linda Jensen, Lone Honoré, and Hans Erik Johnsen

Autologous human plasma in stem cell culture and cryopreservation in the creation of a tissue-engineered vascular graft Ping Zhang, PhD, Aleksandra Policha,

Autocrine release of interleukin-9 promotes Jak3-dependent survival of ALK+ anaplastic large-cell lymphoma cells by Lin Qiu, Raymond Lai, Quan Lin, Esther.

Rapamycin is an effective inhibitor of human renal cancer metastasis1

Autologous human plasma in stem cell culture and cryopreservation in the creation of a tissue-engineered vascular graft Ping Zhang, PhD, Aleksandra Policha,

AKR1C3 is a biomarker of sensitivity to PR-104 in preclinical models of T-cell acute lymphoblastic leukemia by Donya Moradi Manesh, Jad El-Hoss, Kathryn.

T cell receptor Vβ (TCR-Vβ) expression in a drug-specific T cell line (TCL) and specificity assay of a TCL of patient P1. T cell receptor Vβ (TCR-Vβ) expression.

by Anupama Narla, Shilpee Dutt, J

IL-18 Regulates Melanoma VLA-4 Integrin Activation through a Hierarchized Sequence of Inflammatory Factors María Valcárcel, Teresa Carrascal, Olatz Crende,

Evidence for Circulating Bone Marrow-Derived Endothelial Cells

An Important Role of VEGF-C in Promoting Lymphedema Development

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D George W. Agak, Min Qin, Jennifer Nobe,

VEGF-B Improves Metabolic Health through Vascular Pruning of Fat

Combining the Multitargeted Tyrosine Kinase Inhibitor Vandetanib with the Antiestrogen Fulvestrant Enhances Its Antitumor Effect in Non-small Cell Lung.

Volume 2, Issue 4, Pages (October 2002)

International Journal of Cardiology

Angiogenesis and vasculogenesis: Inducing the growth of new blood vessels and wound healing by stimulation of bone marrow–derived progenitor cell mobilization.

Volume 7, Issue 4, Pages (October 2016)

Fibronectin promotes VEGF-induced CD34+ cell differentiation into endothelial cells Errol S Wijelath, PhD, Salman Rahman, PhD, Jacqueline Murray, PhD,

An Autocrine Loop Mediates Expression of Vascular Endothelial Growth Factor in Human Dermal Microvascular Endothelial Cells Barbara Vega-Diaz, Serge.

Volume 109, Issue 5, Pages (May 2002)

Volume 143, Issue 6, Pages e2 (December 2012)

VEGF-B Improves Metabolic Health through Vascular Pruning of Fat

Fig. 2. Circulating VEGF in GCA patients up-regulates microvascular endothelial Jagged1. Circulating VEGF in GCA patients up-regulates microvascular endothelial.

Myeloma cell–derived Runx2 promotes myeloma progression in bone

Defective negative regulation of Toll-like receptor signaling leads to excessive TNF-α in myeloproliferative neoplasm by Hew Yeng Lai, Stefan A. Brooks,

Volume 23, Issue 1, Pages (January 2015)

Two distinct CXCR4 antagonists mobilize progenitor cells in mice by different mechanisms by Andia N. Redpath, Moïra François, Suet-Ping Wong, Dominique.

Michael Boyer, MD, Laurace E. Townsend, PhD, L

Volume 6, Issue 4, Pages (October 2004)

Volume 34, Issue 4, Pages (April 2011)

Human CD4+ T Lymphocytes with Remarkable Regulatory Functions on Dendritic Cells and Nickel-Specific Th1 Immune Responses Andrea Cavani, Francesca Nasorri,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Jun Asai, Hideya Takenaka, Norito Katoh, Saburo Kishimoto

Volume 12, Issue 4, Pages (October 2005)

Volume 11, Issue 5, Pages (November 2018)

Volume 85, Issue 1, Pages (January 2014)

Suppression of VEGFR2 Expression in Human Endothelial Cells by Dimethylfumarate Treatment: Evidence for Anti-Angiogenic Action Markus Meissner, Monika.

VE-cadherin protein and mRNA are decreased in the retinal vessels of diabetic rats. VE-cadherin protein and mRNA are decreased in the retinal vessels of.

NOX1-deficient mice do not respond to GKT771 and exhibit reduced B16-F10 tumor growth associated with decreased production of angiogenic factors and minor.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

Increase in proliferation and activation of immune cells in peripheral blood after NKTR-214 treatment. Increase in proliferation and activation of immune.

Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. LNCaP,

Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.

A, VEGFR2 staining in the tumor rim and center.

Increased tumorigenic activities of TIM-3–expressing RCC cells.

Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte-macrophages in humans by Asako Sawano,

Presentation transcript:

Vascular Trauma Induces Rapid but Transient Mobilization of VEGFR2+AC133+ Endothelial Precursor Cells by Muhammad Gill, Sergio Dias, Koichi Hattori, Mary Lee Rivera, Daniel Hicklin, Larry Witte, Leonard Girardi, Roger Yurt, Harvey Himel, and Shahin Rafii Circulation Research Volume 88(2):167-174 February 2, 2001 Copyright © American Heart Association, Inc. All rights reserved.

VEGF levels are rapidly increased in burn and CABG patients. VEGF levels are rapidly increased in burn and CABG patients. Plasma VEGF levels in burn (A) and CABG (B) patients were determined by ELISA. There was a rapid elevation (6 to 12 hours) of VEGF levels after trauma, followed by a gradual decrease over time (n=5 for burn, n=4 for CABG). Results shown are mean±SD for plasma VEGF levels, in both burn and CABG patient samples. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Expression of VEGFR2 and VE-cadherin mRNA in mobilized PBMNCs by RT-PCR. Expression of VEGFR2 and VE-cadherin mRNA in mobilized PBMNCs by RT-PCR. Representative sample from a burn (A) and CABG (B) patient, showing VEGFR2 (KDR) and VE-cadherin mRNA expression as determined by RT-PCR analysis of the PBMNCs. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Quantification of the mobilized VEGFR2+ and VE-cadherin+ cells by flow cytometry. Quantification of the mobilized VEGFR2+ and VE-cadherin+ cells by flow cytometry. Representative two-color flow cytometry analysis of mobilized PBMNCs, quantifying the number of VEGFR2+ and VE-cadherin+ cells 12 hours after burn injury (A) and 6 hours after CABG (B), demonstrating the number of VEGFR2+ (5.3% in burn, 11.1% in CABG) and VE-cadherin+ (4.7% in burn, 8.7% in CABG) cells. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Time course of the total number of mobilized VEGFR2+CD15− cells in the PBMNCs of burn and CABG patients. Time course of the total number of mobilized VEGFR2+CD15− cells in the PBMNCs of burn and CABG patients. The total number of mobilized VEGFR2+CD15− cells over time in burn (n=8) (A) and CABG (n=7) (B) patients was determined by two-color flow cytometry in 1 mL of peripheral blood. Nonviable cells were excluded by PI staining. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Early and late endothelial outgrowth colony formation by the circulating mononuclear cells from burn (A) and CABG (B) patients. Early and late endothelial outgrowth colony formation by the circulating mononuclear cells from burn (A) and CABG (B) patients. PBMNCs (105 cells) obtained from the peripheral blood were cultured on gelatin-coated plates in the presence of endothelial growth medium. Adherent proliferating colonies that formed in the first 2 weeks and late colonies that formed in the passaged cells during 6 to 8 weeks of initial culture were quantified for the metabolic incorporation of DiI-Ac-LDL (red) as well as vWF (green) staining (C). DiI-Ac-LDL+vWF+-adherent colonies that formed from passaged nonadherent cells, between 6 to 8 weeks of initial culture, were scored as late-outgrowth colonies (CFU-ECs), whereas those that formed from initial plating, within the first 3 weeks, were considered as early outgrowth colonies. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Acute VEGF elevation promotes rapid but transient mobilization of VEGFR2+ cells. Acute VEGF elevation promotes rapid but transient mobilization of VEGFR2+ cells. A, Two-color flow cytometry showing the number of VEGFR2+CD11b− cells mobilized 24 hours after AdNull or AdVEGF165 intravenous injections, demonstrating that 1.7% and 14.7% of VEGFR2+CD11b− cells were mobilized, respectively. B, Time course of the mobilized VEGFR2+CD11b− cells in the AdNull- and AdVEGF165-injected mice (n=3). The number of VEGFR2+CD11b− cells was determined by flow cytometry. Nonviable cells were excluded by PI staining. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.

Rapid elevation of VEGF in mice by injection of AdVEGF165. Rapid elevation of VEGF in mice by injection of AdVEGF165. Plasma VEGF levels in the mice injected with AdVEGF165 and AdNull vectors were determined by ELISA. There was a rapid elevation (24 hours) of VEGF levels after AdVEGF165 injection, followed by a gradual decrease over time (n=3). Results are the mean±SD for plasma VEGF levels. Muhammad Gill et al. Circ Res. 2001;88:167-174 Copyright © American Heart Association, Inc. All rights reserved.