Arterioscler Thromb Vasc Biol

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Arterioscler Thromb Vasc Biol A Highly Efficient Method to Differentiate Smooth Muscle Cells From Human Embryonic Stem Cells by Chang-Qing Xie, Jifeng Zhang, Luis Villacorta, Taixing Cui, Huarong Huang, and Y. Eugene Chen Arterioscler Thromb Vasc Biol Volume 27(12):e311-e312 December 1, 2007 Copyright © American Heart Association, Inc. All rights reserved.

Figure. Characterization of SMCs derived from hES cells. Figure. Characterization of SMCs derived from hES cells. A, Immunohistochemical staining of SMC-like cells derived from hES cells with SMC-specific markers, SMMHC (left panel, green) and SM-α-actin (right panel, red). The nuclei (blue) were costained with DAPI. Bar=100 μm. B, Representative Western blots showing SMC-specific proteins including SMMHC, SM-α-actin, and caldesmon. β-tubulin was used as the loading control. C, Carbachol-induced contraction of SMC-like cells differentiated from hES cells. Photographs were taken before (left panel) and after (right panel) treatment with 1 mM carbachol for 10 minutes. Arrowheads show representative contracted cells grown in differentiation conditions. Bar=200 μm. Chang-Qing Xie et al. Arterioscler Thromb Vasc Biol. 2007;27:e311-e312 Copyright © American Heart Association, Inc. All rights reserved.