Biotechnological Research on Endophytic Microbes

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Presentation transcript:

Biotechnological Research on Endophytic Microbes from a tea Plant and the Parasitic Plant Partomuan Simanjuntak Research Center for Green Science Fukuyama University Research Centre for Biotehnology, Indonesian Institute of Sciences (LIPI)

Collected at the Puncak area, West Java, Indonesia

tea plant

endophytic fungus tea plant

biotransformed product (6) 500.00 absorbance (+)-catechin (1) 0.00 0.00 10.00 20.00 30.00 40.00 time (min)

biotransformed product (7, 39%) 500.00 biotransformed product (7, 39%) absorbance (-)-epicatechin (2, 2.4%) 0.00 0.00 10.00 20.00 30.00 40.00 time (min)

biotransformed product (7, 53%) 200.00 biotransformed product (7, 53%) absorbance (-)-ECG (4) (-)-epicatechin (2, 13%) 0.00 0.00 10.00 20.00 30.00 40.00 time (min)

biotransformed product (8, 43%) 200.00 biotransformed product (8, 43%) absorbance (-)-epigallocatechin (3, 19%) (-)-EGCG (5) 0.00 0.00 10.00 20.00 30.00 40.00 time (min)

Collected at the Puncak area, West Java, Indonesia

(+)-catechin

Separation

a new fungus, Phomopsis sp. Fungus H, a new fungus, Phomopsis sp.

(+)-catechin

- The microorganism is always right, your friend and sensitive partner Law of Applied Microbiology - The microorganism is always right, your friend and sensitive partner - They are no stupid microorganism - Microorganisms can (will) do anything - Microorganism are smarter, wiser, more energetic than chemist, microbiologist, etc - If you take of your (microbial) friends, they will take care of your future (and you will live happily everyafter) Law of Applied Microbiology - The microorganism is always right, your friend and sensitive partner - They are no stupid microorganism - Microorganisms can (will) do anything - Microorganism are smarter, wiser, more energetic than chemist, microbiologist, etc - If you take of your (microbial) friends, they will take care of your future (and you will live happily everyafter) (D. Parlman, 1979)

The Puncak area, West Java, Indonesia

ありがとう ございます Terima Kasih

Symbiosis relationship between plant and endophytic microbes Microbes which associates with living tissue of the plant. The type of interaction between endophyte and its host plant usually mutualism symbiosis. The plant providing nutrients for microbe, while the microbe transform and produce bioactive compounds

Chemical compounds of mahkota dewa, LIPI Chemical compounds of mahkota dewa, Phaleria macrocarpa Bioactive compound

Produksi mikroba endofit C. aeruginosa (Jepang) Produksi mikroba endofit C. domestica/C.longa (Indonesia)