(a) (b) Figure S1. Amino acid sequence alignment of human and pig FSD2. The amino acid sequence of porcine FSD2 (determined in this study), is aligned.

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(a) (b) Figure S1. Amino acid sequence alignment of human and pig FSD2. The amino acid sequence of porcine FSD2 (determined in this study), is aligned to that from (a) pig (Sus scrofa, Genbank accession no.XP_005666169.1) and (b) human (Homo sapiens, Genbank accession no.NP_001007123.1). Identical amino acids are presented with a gray background. The red box indicates the additional amino acids in porcine FSD2.

TGGGAAACCC CAGAGGTGTA TACAAGCTTA GAGGGATAAA CAAAGCTCTA AGCATCTGCC TGCCACAAAG GGCTAGAAGC TAGCATCATC TGTGGCACCA GCACACATAG GAGCTGTCTT ATCACCTTAA CGCCTCCTGC CTGTGGCAGA CAAGCCAGAG CTCAGGGAGA AAGTGTGGGA AGAAGAGCGC CACCTCATCC CTCGTTTTTC AGGGCAGACT TCCAGCTCCA GAGGCCTAAG CTGGGAAGGG GAGAAGGTTT CAATGCTGGC TAAGCTAAAT TTGACTTTTT AGTGAATGTA CTGGACTGGA CTGTATTACC AAATGGAGAC TATGCCTTCA AGTGTGTTAC CTAAGAGGGA GCAGAAGGTC ATGGGTCTGC TGAATTTTTT TTTTTTTAAT CTTTTGAGGG CCCACCCATG GCATATGGAG GTTCCCAGGC TAGGGATTGA AGTGGAGCTG CAGCCGCCGG TCCACACCAC AGCCACAGCA ACGCCAGATC TGAGCTGAAT CTGCAACCCA CACCACATCC CATGGCAAGG TTGGATCCCC CACTCACTGA GTGAGGTCAG GGATTGAACC GGTGTCCCCA TGGATACCAG TTTCTGCTGA GCCACAAGGG GAACTCCAGT CTGCTGGATT TTTCTCCAGA GAGGGGAGTA ATTCCTGCCC CTCAGGCCAG GTGGAAGGTG CACAGCATTT GGCCTCTCAG AGTCCATTCT CTTGTGTGGC CTGCAGCTGC CCTCCCAACC TCACAGTGAC AGCCCCCTGC CCTCTACACA TGCCCAGCCA CCTGAGTCCC TGGGTAGAGC CACAGCACTC AAATCCCCAC AGTAAATGAA GTAACATTTA TTACATGTGA CCTACCCTGG TGACACTTGA GCAGTAAGCA GTGGCAGGTC TATATTTGGG CTTCCACTCA GGCAGGCCTG TTGCTATTTG CAGCCAGAGC CAGCTCGTGT TACCCCTGCC TTTGCTGCCC CACTTGTAGG ACTCTGGGGT AGAACAGGCA GGGGCAGCCT GCCGGGCGTA CAGAGGTCAC - 1000 - 920 myogenin - 840 myogenin - 760 - 680 MyoD myogenin - 600 myogenin myogenin - 520 - 440 MyoD myogenin MyoD myogenin - 360 myogenin - 280 myogenin MyoD - 200 Nkx-2.5 - 120 myogenin - 40 Nkx-2.5 exon 1 → Figure S2. Putative transcription factor binding sites in the 1,000-bp region upstream of porcine FSD2 related to tissue-specific expression. Green and red boxes indicate transcription factor binding sites identified using ALGGEN-PROMO and TRANSFAC, respectively.

Arg333 Gly333 FN3 FN3 FN3 FN3 Figure S3. The predicted protein structure of porcine FSD2 derived from amino acid residues 296 to 561. The substitution of Arg (a) in haplotype 1 to Gly (b) in haplotype 2 at position 333 is located near SNP10 (marked in purple). The porcine FSD2 structure of helix and fibronectin type III (FN3) domains are represented in red and sky-blue, respectively.