Effect of concentration of Halo-FGF1 and of Halo-FGF2 on their binding and diffusion in Rama 27 fibroblast pericellular matrix. Effect of concentration.

Slides:

Advertisements

Similar presentations

P247. Figure 9-1 p248 Figure 9-2 p251 p251 Figure 9-3 p253.

Advertisements

Farmer et al Supplementary Figure 1 KU PARP-1 IC 50 = 3.2nM KU PARP-1 IC 50 = 3.4nM KU PARP-1 IC 50 = 730nM a b

Thrombospondin 1 as a scavenger for matrix-associated fibroblast growth factor 2 by Barbara Margosio, Daniela Marchetti, Veronica Vergani, Raffaella Giavazzi,

Measurement of the p38 signaling pathway in the Pro253Arg mutation of FGFR2 in Apert syndrome Kaivalya Dandamudi.

Mental Math Activity.

Title of Your Research Heading 3 Heading 6 Heading 4 Heading 1

Plot of PC1 of femur bone measurements from mainland emus, Kangaroo Island emus, and King Island emus versus natural log of island size. Plot of PC1 of.

- PARTING KNOWLEDGE ABOUT FRACTIONAL NUMBERS

Janina Jamasbi et al. BTS 2016;1:

Authors’ names to be inserted here, one or two lines in total

Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

Figure 4 Model of changes in the serum levels

Prediction of IGHV status based on Factor 1 in the CLL data and validation on outlier cases on independent assays Prediction of IGHV status based on Factor.

Steven E. Williams et al. JACEP 2017;j.jacep

Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.

Supplementary Figure 1 A. B..

Label Name Label Name Label Name Label Name Label Name Label Name

Supplemental Figure 1A – I

The Plasticity of the Hsp90 Co-chaperone System

Janina Jamasbi et al. BTS 2016;1:

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Authors’ names to be inserted here, one or two lines in total

Allosteric site of B. subtilis PRPP synthase.

Peroxisomal biogenesis deficiency in skin fibroblasts from a patient with PEX14 mutation. Peroxisomal biogenesis deficiency in skin fibroblasts from a.

Janina Jamasbi et al. BTS 2016;1:

Titrations of other division proteins support FtsZ as the division limitation Titrations of other division proteins support FtsZ as the division limitation.

Fibroblast proliferation and ECM changes during wound healing

Janina Jamasbi et al. BTS 2016;1:

Haguy Wolfenson, Irena Lavelin, Benjamin Geiger Developmental Cell

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Translation dynamics of single mRNAs in live cells and neurons

ChIP-seq analyses in primary endometrial tissue.

Daniel Schultz, Adam C. Palmer, Roy Kishony Cell Systems

Peptide concentration controls digital NFAT1 nuclear translocation kinetics in a responder fraction of stimulated naive OT-I cells. Peptide concentration.

Cohesin Can Remain Associated with Chromosomes during DNA Replication

Binding competition between B2′B3 and B3 fragments of TcdB.

The B2′B3 region confers the binding phenotype of full-length TcdB.

C.2.10 Sample Questions.

In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.

Volume 2, Issue 6, Pages (December 2012)

C.2.8 Sample Questions.

C.2.8 Sample Questions.

FRAP analysis of Cdt2 binding to sites of damage in the absence of Cdt1. FRAP analysis of Cdt2 binding to sites of damage in the absence of Cdt1. (A) Quantitative.

Plk1 inhibition affects the NuMA turnover at the spindle pole.

Oscillations in isotopic labeling of TCA cycle metabolites throughout the cell cycle from [U‐13C]‐glucose show induced glycolytic flux into TCA cycle in.

Aortic Vasorelaxation at 18 Weeks Post TAC Freshly isolated thoracic aortic rings were allowed to equilibrate, and pre-contracted with 1 μM phenylephrine.

Volume 98, Issue 1, Pages (January 2010)

Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.

Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin.

Inhibition of FL accumulation by glutathione and a size range of its conjugates. Inhibition of FL accumulation by glutathione and a size range of its conjugates.

Figure 2 C5B3 prevented AQP4-IgG–mediated CDC without affecting AQP4-IgG binding to AQP4 C5B3 prevented AQP4-IgG–mediated CDC without affecting AQP4-IgG.

Fluorescence properties of MSP.

The FRAP mobility of EGFP-UAP56, but not its K95N mutant, is ATP-dependent. The FRAP mobility of EGFP-UAP56, but not its K95N mutant, is ATP-dependent.

Manfred Frick, Katja Schmidt, Benjamin J. Nichols Current Biology

Effect of FGF8 on R-C marker expression.

Figure Serial brain MRI of the patient with encephalitis and spontaneous recovery accompanying IgLON5 autoimmunity Serial brain MRI of the patient with.

SpC3 concentrations in coelomic fluid (CF) collected from sea urchins at various times before and after the injection of LPS or ASW. Data are shown as.

Interaction of full-length TcdB with cells.

Fig. 4 EUV TG signal from Si.

Fig. 3 Correlation between magnetoresistance and emergence of superconductivity. Correlation between magnetoresistance and emergence of superconductivity.

Pulse chase analysis of the synthesis, secretion, proteolytic processing, and extracellular matrix (ECM) incorporation of fibrillin by 2562 and an age.

Estimating statistical significance using the overlap rule for 95% CI bars. Estimating statistical significance using the overlap rule for 95% CI bars.

Exocytosis of MVEs releases exosomes containing transferrin receptor.

Thy1S promoter drives sparse expression of hrGFP in marmoset cortex.

Differentiation of common pediatric brain tumors by quantitative 1H-MR spectroscopy. Differentiation of common pediatric brain tumors by quantitative 1H-MR.

Competition by G3139 for binding of ClRNH32P-OdT18 to collagen I (A), FGF2 (B), PDGF BB (C), and VEGF (D). Competition by G3139 for binding of ClRNH32P-OdT18.

Fig. 1 Fractional coverage of the mapping method used in this study.

Group trajectories of change in serial WHIM ratings categorised by PDOC status at discharge. Group trajectories of change in serial WHIM ratings categorised.

Comparison of 16S sequencing and shallow shotgun recovery of species-level taxa. Comparison of 16S sequencing and shallow shotgun recovery of species-level.

Fig. 4 Matrix for intersections of differentially expressed transcripts between RNAiAFL transgenic and null kernels. Matrix for intersections of differentially.

Presentation transcript:

Effect of concentration of Halo-FGF1 and of Halo-FGF2 on their binding and diffusion in Rama 27 fibroblast pericellular matrix. Effect of concentration of Halo-FGF1 and of Halo-FGF2 on their binding and diffusion in Rama 27 fibroblast pericellular matrix. TMR-Halo-FGF1 (1 nM) was used to label fixed Rama 27 fibroblasts to identify any dependence of their level of binding and their diffusion on concentration. The FRAP experiments with TMR-Halo-FGF1 (2 nM) and TMR-Halo-FGF2 (2 nM) were carried out at the same time and are shown in figures 7 and 8. Six FRAP experiments are included for each sample. (a) Binding intensities of Halo-FGF1 (1 and 2 nM) and Halo-FGF2 (2 nM) at the areas for FRAP measurements. (b) The moving speed (half recovery time) of different concentration of Halo-FGF1 and Halo-FGF2 in the matrix. (c) The mobile/immobile fraction (Final recovery level) of different concentration of Halo-FGF1 and Halo-FGF2 in fixed Rama 27 fibroblasts pericellular matrix. Changye Sun et al. Open Biol. 2016;6:150277 © 2016 The Authors.