Genetic Engineering Insulin production Extra-nutrient foods

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Genetic Engineering Insulin production Extra-nutrient foods Sickle cell anemia treatment Insulin production Extra-nutrient foods

Enter: BACTERIA -Nucleoid -Plasmid- small circular DNA that replicates separately  -Contains only a few genes -Need to amplify and clone DNA sequences  -If want to see result need to have a lot of a single gene

-DNA cloning: making more copies of a DNA sequence  -Isolate a plasmid (cloning vector)  -Insert new gene (recombinant DNA)  -Put plasmid back into bacteria  -Grow bacteria and harvest

Restriction Enzymes -Enzymes that cut up DNA in specific locations  -Found in bacteria and archaea  -Defense against viruses  -Cut up foreign DNA in a process called restriction Over 3000 identified!

wikipedia.org

Recombinant DNA Cloning vector