Genetic Engineering Insulin production Extra-nutrient foods Sickle cell anemia treatment Insulin production Extra-nutrient foods
Enter: BACTERIA -Nucleoid -Plasmid- small circular DNA that replicates separately -Contains only a few genes -Need to amplify and clone DNA sequences -If want to see result need to have a lot of a single gene
-DNA cloning: making more copies of a DNA sequence -Isolate a plasmid (cloning vector) -Insert new gene (recombinant DNA) -Put plasmid back into bacteria -Grow bacteria and harvest
Restriction Enzymes -Enzymes that cut up DNA in specific locations -Found in bacteria and archaea -Defense against viruses -Cut up foreign DNA in a process called restriction Over 3000 identified!
wikipedia.org
Recombinant DNA Cloning vector