DATA ANALYSIS.

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Restriction Analysis of Plasmids

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Presentation transcript:

DATA ANALYSIS

L 2 3 4 5 6 7 8

DNA Ladder for Mapping of Plasmid DNA: BEFORE adding ladder heat at 65C for 2 minutes 23,130 bp 9416 bp 6556 bp 4361 bp 3000 bp [brighter than other bands!] 2322 bp 2027 bp 725 bp 570 bp

You will collect data and determine: Location of cutting sites Present the “map” of the plasmid in your report

Data will be obtained from your photo DNA Fragments various sizes: Ladder “M”

Standard Curve Measure distance migrated of known sizes Versus DNA known sizes Graph Measure distance migrated of unknown

Graph Log Scale: Standard Curve This is from your ladder DNA (bp) Distance (cm)