Supplementary FigureS4 (Feduska et al.)

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Supplementary FigureS4 (Feduska et al.) B Control E-cadherin Occludin Vimentin Snail 1 GAPDH WT mAB4 mAB8 SK-N-AS SK-N-AS Control WT mAB4 mAB8 GAPDH c-Myc FigureS4. Expression of RNA encoding stemness and EMT markers was similar in control SK-N-AS cells and ICAM-2 WT transfectants. No differences were seen in levels of RNA encoding c-Myc (stemness marker) S1, E-cadherin, Occulidin, Vimentin, or Snail 1 (EMT markers) S2 among the four SK-N-AS NB cell transfectants. Primers used for RT-PCR analysis were: E-cadherin (forward) 5’- CAGCACGTACACAGCCCTAA-3’ and (reverse) 5’- ACCTGAGGCTTTGGATTCCT-3’; Occludin (forward) 5’GATGACTTCAGGCAGCCTCG-3’ and (reverse) 5’- CTATGTTTTCTGTCTATCATAGTC-3’; Snail 1 (forward) 5’- GAAAGGCCTTCAACTGCAAA-3’ and (reverse) 5’-TGACATCTGAGTGGGTCTGG-3’; c-Myc (forward) 5'- CGACTCTGAGGAGGAACA AG-3‘ and (reverse) 5'- GTGATCCAGACTCTGACCTTT-3’; GAPDH (forward) 5’- AACATCATCCCTGCT-TCCAC-3’ and (reverse) 5’- GACCACCTGGTCCTCAGTGT-3’. S1Bandopadhayay P, Bergthold G, Nguyen B, Schubert S, Gholamin S, et al. BET- bromodomain inhibition of MYC-amplified medulloblastoma. Clin Cancer Res. 2013. S2Moon H-J, Finney J, Xu L, Moore D, Welch DR, Mure M. MCF-7 cells expressing nuclear associated lysyl oxidase-like 2 (LOXL2) exhibit an epithelial-to-mesenchymal transition (EMT) phenotype and are highly invasive in vitro. J Biol Chem 2013; 288: 30000-30080.