Arterioscler Thromb Vasc Biol T-786C Polymorphism in Endothelial NO Synthase Gene Affects Cerebral Circulation in Smokers by Shampa Nasreen, Toru Nabika, Hiroshi Shibata, Hidehiko Moriyama, Kazuya Yamashita, Junichi Masuda, and Shotai Kobayashi Arterioscler Thromb Vasc Biol Volume 22(4):605-610 April 1, 2002 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Allele-specific PCR for T-786C polymorphism in the eNOS gene. Figure 1. Allele-specific PCR for T-786C polymorphism in the eNOS gene. Top panel shows the primers used in the reaction and the expected PCR products. Underlining in the primer sequences indicates artificially introduced mismatches. Bottom panel shows a typical result of genotyping. Shampa Nasreen et al. Arterioscler Thromb Vasc Biol. 2002;22:605-610 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. Effects of T-786C genotype on cerebral circulation in smokers and nonsmokers. Figure 2. Effects of T-786C genotype on cerebral circulation in smokers and nonsmokers. Effects of T-786C genotype of the eNOS gene on CBF, CVR, and MBP were examined in smokers and in nonsmokers. Each column and line indicates a mean and SD, respectively. *P<0.05 vs TC and TT by ANOVA. Shampa Nasreen et al. Arterioscler Thromb Vasc Biol. 2002;22:605-610 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Effects of age on CBF in the smokers with TC and TT genotypes. Figure 3. Effects of age on CBF in the smokers with TC and TT genotypes. A stepwise exclusion of younger subjects was performed in TC (shaded bars) and TT (open bars) genotypes. Although the distribution of age went up with the stepwise exclusion (bottom panel), CBF did not change (top panel). *P<0.05 among CC (of all ages, hatched boxes), TC, and TT by ANOVA. Shampa Nasreen et al. Arterioscler Thromb Vasc Biol. 2002;22:605-610 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Effects of smoking and T-786C eNOS genotype on plasma NOx Figure 4. Effects of smoking and T-786C eNOS genotype on plasma NOx. Plasma NOx was measured as described in Methods. Figure 4. Effects of smoking and T-786C eNOS genotype on plasma NOx. Plasma NOx was measured as described in Methods. Each column and line indicate a mean and SD, respectively. Solid and open bars represent nonsmokers and smokers, respectively. Shampa Nasreen et al. Arterioscler Thromb Vasc Biol. 2002;22:605-610 Copyright © American Heart Association, Inc. All rights reserved.