EMT correlates with PERK but not IRE1 signaling in primary human tumors. EMT correlates with PERK but not IRE1 signaling in primary human tumors. A, two.

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EMT correlates with PERK but not IRE1 signaling in primary human tumors. EMT correlates with PERK but not IRE1 signaling in primary human tumors. A, two primary breast cancer cells (BT5104 and BT5094) were freshly collected from patient ascites, and cell lysates were collected and analyzed by Western blotting for differentiation markers. B, Western blot analysis of non-EMT–like BT5104 cells and EMT-like BT5094 cells treated with 0, 5, and 10 nmol/L of thapsigargin for 2 days for expression of PERK, BiP, phospho-eIF2α (p-eIF2α), and β-actin. C, dose–response curves of non-EMT–like BT5104 and EMT-like BT5094 cells treated with increasing concentrations of tunicamycin or thapsigargin for 3 days. Cell survival was determined using an ATP-based luminescence assay. Data are represented as mean +/- SD from three replicates. D, correlation analyses of expression of EMT genes and ECM genes, XBP1-targeted genes, or ATF4-targeted genes in breast cancers (GSE41998; n = 255), colon cancers (GSE37892; n = 130), gastric cancers (GSE26942; n = 90), lung cancers (GSE4573; n = 130), and metastatic cancers of various origins (GSE11360; n = 187). Yu-xiong Feng et al. Cancer Discovery 2014;4:702-715 ©2014 by American Association for Cancer Research