The first European cave fish Jasminca Behrmann-Godel, Arne W. Nolte, Joachim Kreiselmaier, Roland Berka, Jörg Freyhof  Current Biology  Volume 27, Issue 7, Pages R257-R258 (April 2017) DOI: 10.1016/j.cub.2017.02.048 Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 1 Cave loaches adapted to life in constant darkness are of recent origin but genetically divergent from surface populations residing in the same drainage. (A) Two cave loaches in their natural habitat. (B) Adult male loach with typical adaptations to living in caves: reduced eyes, enlarged barbels and pale body coloration. (C) Typical epigean loach from the surface population in the Danube. (D) Neighbour-joining tree of Cytochrome oxidase subunit1 (COI) sequences for European stone loaches. European loaches form three clusters comprising sequences from Rhine, Danube and Elbe drainages. Loaches from the Danube–Aach cave system (AC, highlighted in orange) share haplotypes with individuals from the southern lineage sampled in the upper Rhine and Danube drainage, individuals from GenBank identified by their accession numbers. Numbers at major nodes indicate bootstrap values (>50%, 1000 replicates). (E) Graphical representation of an analysis of genetic population structure. Inferred genomic ancestry in three genetic clusters (y-axis) is depicted by red, green and blue colors for all individuals (x-axis). With prior information on the sample location of individuals, three clearly distinct genetic clusters separate cave loaches from both surface populations upstream (Danube) and downstream (Radolfzeller Aach) in the same drainage. Without prior information on the sample location of individuals, cave fish are grouped with the upstream but clearly separated from the downstream loach population. Current Biology 2017 27, R257-R258DOI: (10.1016/j.cub.2017.02.048) Copyright © 2017 Elsevier Ltd Terms and Conditions