Streptococci Basmah almaarik

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Upper and Lower Respiratory Tract Infection

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Presentation transcript:

Streptococci Basmah almaarik Lecture # 5b

Streptococci All are Gram Positive Cocci arranged in chains (short or long) All are Catalase -ve + ve - ve

Gram Stain Staphylococci streptococci

Culture Fastidious organism  no growth on NA Grows well on BAP Facultative anaerobe Grow at 37C need 24 to 48 hour .

Streptococcus Classification Alpha hemolytic S. viridans S. pneumoniae Beta hemolytic: Group A, B, C, G & D Lancefield grouping according to antigens in their cell wall Non-hemolytic: Enterococcus (formally known as Group D)

Alpha Hemolytic Streptococci Give alpha hemolysis on BAP. It Can be divided in to 2: Viridans group S.pneumoniae

Streptococcus viridans s. pneumoniae Streptococcus viridans

Viridans Streptococci S. PNEUMONIAE Alpha haemolysis Pin point colonies Optochin  resistant Bile Solubility  -ve Part of normal flora of upper respiratory and GI Alpha haemolysis Ring form colonies Tanslucent or mucoid Optochin  sensitive Sile solubility  +ve Not part of NF Not Capsulated Flattened with raised edges Capsulated

Viridans Streptococci S. PNEUMONIAE GPS in chains GPC elongated (Lanceolated) diplococci

On the primary culture of isolation plate before incubation 1. Optochin Test Ethylhydrocupreine hydrochloride. Zone of inhibition of 14 mm or more  sensitive  S. pneumoniae No zone of inhibition  S.viridans Always don on a BAP. On the primary culture of isolation plate before incubation

Optochin Disk

. Bile Solubility Test To test if colonies are lysed by bile salt (such as desoxycholate) or not. There are 2 ways to do the test : In a tube On plate

Bile Solubility in Tube 2 ml of saline in a tube Emulsify several colonies of test organism in it Divide organism suspension in 2 tubes Add 2 drops of bile salt to one tube and 2 drops of sterile distilled water to the other. Leave 10-25 mn at RT

Bile Solubility on Plate Add drop of bile salt to specified part of plate Incubate at 37C for 10 - 30 min Colony disappear  +ve Colony stay  -ve

Rapid Detection of S. pneumoniae Rapid latex and coagglutination tests are available to detect capsular pneumococcal antigen in CSF or other sterile body fluids.

Sensitivity Testing of S. pneumoniae

Thank You

Your work today: Do gram stain for S.viridance and S.pnemoniae (show it to the instructor) Streak blood agar with S.viridance and S.pnemoniae and inoculate both organisms with Optochin disk.