Tamoxifen induced deletion of FAK

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Tamoxifen induced deletion of FAK Tamoxifen induced deletion of FAK. A, Genomic DNA of wild-type (WT) and Cre-positive floxed FAK mice were amplified by PCR. The WT band is 1.4 kb, the floxed FAK product is 1.6 kb and the postrecombination product is 550 bp. Tamoxifen induced deletion of FAK. A, Genomic DNA of wild-type (WT) and Cre-positive floxed FAK mice were amplified by PCR. The WT band is 1.4 kb, the floxed FAK product is 1.6 kb and the postrecombination product is 550 bp. DNA was extracted from cardiac tissue unless otherwise noted. FAK C, Cre-positive floxed-FAK mouse not treated with tamoxifen; Sk M, skeletal muscle. B, Representative Western blot showing FAK protein expression in lysates of isolated adult cardiac ventricular myocytes from wild-type (WT), experimental control (Exp C), and FAK KO mice. C, Normalized integrated intensity data for myocyte FAK expression in WT, Exp C and FAK KO mice. FAK expression in FAK KO mice was reduced by 66.5% compared to WT mice (P≤0.001; n=7 hearts, both groups) and by 73% compared to Exp C mice (P≤0.001; n=5 hearts [Exp C], n=7 hearts [FAK KO]). Numbers within bars indicate sample size of each group. Exp C indicates experimental control; FAK, focal adhesion kinase; KO, knock out; PCR, polymerase chain reaction. Adam J. Perricone et al. J Am Heart Assoc 2013;2:e000457 © 2013 Adam J. Perricone et al.