OUTLINE 2 II. Cell Differentiation A. What do we know?

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OUTLINE 2 II. Cell Differentiation A. What do we know? B. The developmental landscape model C. Two hypotheses for how cells become differentiated 1. Nuclear alteration 2. Nuclear differentiation Experimental evidence 1. Seward totipotency in carrots 2. Gurdon - nuclear transplantation in toads

Your TAs Eric Raab: gonoles33@aol.com Nicole Stevens: nms02f@garnet.fsu.edu You Class Major Career Fr: 9 Bio: 128 Medicine: 95 So: 147 Ex. Phys: 56 Allied Health: 48 Jr: 57 Biochem: 16 Research: 21 Sr: 27 Chem: 10 Vet med: 12 Psych: 9 Other: 6 Undecided: 58

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Outline of Lecture 1 I. THE CENTRAL DOGMA A. DNA structure B. DNA Replication C. Chromosomes 1. Prokaryotic 2. Eukaryotic D. RNA Structure E. Transcription 1. Summary 2. Prokaryotes 3. Eukaryotes F. Translation 1. The genetic code 2. Summary of translation

Fig. 17.4 The Genetic Code Codon - 3 base sequence that codes for one amino acid or is a signal.

Fig. 17.13 Transfer RNA (tRNA)

Fig. 17.12 Translation of mRNA into protein

Fig. 17.25

Muscle Intestinal Blood Nerve EGG

OUTLINE 2 II. Cell Differentiation A. What do we know? B. The developmental landscape model C. Two hypotheses for how cells become differentiated 1. Nuclear alteration 2. Nuclear differentiation Experimental evidence 1. Seward’s totipotent carrots 2. Gurdon - nuclear transplantation in toads

Waddington’s Developmental Landscape Undifferentiated cell muscle lung brain skin

Which One? Nuclear Alteration Nuclear Differentiation

Fig. 21.5 Seward’s carrot experiment

Nuclear transplantation Fig. 21.6

Issues related to totipotency Stem cell research Fig. 21.8 Dolly and “mom” Noah - an endangered guar

OUTLINE 3 Control of Gene Expression in Prokaryotes A. Regulatory proteins B. The operon model C. Examples 1. the lac operon (substrate induction) 2. the tryp operon (end product repression) 3. the lac operon (positive control)