Source: Color Atlas of Medicine and Parasitology. 1977 Wuchereria bancrofti MORPHOLOGY ADULT MALE 40 x 0,1 mm, yellowish white, cylinder, slender, Caudal part bends to ventral portion Source: Color Atlas of Medicine and Parasitology. 1977 W. Peters & H.M. Gillers

Wuchereria bancrofti MORPHOLOGY ADULT Sumber : Color Atlas of Medicine and Parasitology. 1977. W. Peters & H.M. Gillers FEMALE (80-100) x (0,24-0,30) mm, soft, blunt nail, slight rounded head.

MORPHOLOGY OF MICROFILARIAE Cephalic space Sheath Slender tail Nuclei Source : Medical Parasitology in Plates. G. Piekarski

Microfilaria Brugia malayi Microfilaria Wuchereria bancrofti Microfilaria Brugia timori

Wucheria bancrofti MORPHOLOGY MICROFILARIAE Source : Color Atlas of Medicine and Parasitology. 1977 W. Peters & H.M. Gillers. Pewarnaan giemsa Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk. Pewarnaan hematoxylin Cephalic space (L:W=1:1) Tail (No nuclei)

Brugia malayi MORPHOLOGY MICROFILARIAE Cephalic space (L:W=2:1) Bloated tail (nucleus in it) B. malayi W. bancrofti

(dr. Djaenudin Natadisastra, SpParK) Brugia timori MORPHOLOGY MICROFILARIAE Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk. Looking like microfilariae B. malayi Size 287-341  Sheathed not clear at coloration of Giemsa (dr. Djaenudin Natadisastra, SpParK)

Brugia timori MORPHOLOGY MICROFILARIAE Stiff body broken, tip of tail rather blunt “Nuclei” in the body, spread in the irregular groups, presence of (5-8) nuclei in the tail Smaller nuclei at the distal than in B. malayi Cephalic space: 3 : 1 Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk.

Plasmodium sp. GENERAL MORPHOLOGY IN ERYTHOCYTIC CYCLES FOUND TWO MAIN STAGE : TROPHOZOITE STAGE Ring form Old/Matured trophozoite SCHIZONT STAGE Young schizont Old/Matured schizont

Plasmodium sp. MORPHOLOGY TROPHOZOITE STAGE Ring Form with reddish Nucleus and bluish Protoplasm Chromatin dots Nucleus Protoplasm ERYTHROCYTE

Nucleus and Protoplasm grow bigger Plasmodium sp. MORPHOLOGY TROPHOZOITE STAGE Nucleus and Protoplasm grow bigger ERYTHROCYTE Nucleus Protoplasm

Plasmodium sp. MORPHOLOGY Nucleus starts fission process SCHIZONT STAGE Nucleus starts fission process Protoplasm remains in size ERYTHROCYTE Nucleus Protoplasm

Plasmodium sp. MORPHOLOGY SCHIZONT STAGE Complete Merozoites Note the Regularity, Pigment ERYTHROCYTE Merozoite

Erythrocyte enlarged, pale PLASMODIUM VIVAX Ring Form Erythrocyte enlarged, pale Schuffner dots ERYTHROCYTE ERYHTROCYTE TROPHOZOITE STAGE

PLASMODIUM VIVAX Protoplasm grows (Amoeboid form) with vacuole TROPHOZOITE STAGE Protoplasm grows (Amoeboid form) with vacuole ERYTHROCYTE Amoeboid Form Vacuole

PLASMODIUM VIVAX Fission of Nucleus Vacuole disappear SCHIZONT STAGE ERYTHROCYTE

PLASMODIUM VIVAX SCHIZONT STAGE Merozoite (12-14) Pigment MEROZOITe

PLASMODIUM VIVAX Round/Oval Round/Oval GAMOTOCYTE STAGE: Macro and Microgametocyte Round/Oval Small nucleus , compact, eccentric Bluish protoplasm NUCLEUS Round/Oval Big nucleus, pale Red-blue protoplasm Pigment NUCLEUS

PLASMODIUM OVALE TROPHOZOITE STAGE Ring Form (thick) Erythrocyte Chromatin dots

PLASMODIUM OVALE Round, compact trophozoite Erythrocyte deformed TROPHOZOITE STAGE Round, compact trophozoite Erythrocyte deformed Schuffner/James’ Dots

Plasmodium malariae TROPHOZOITE STAGE Resemble P. vivax Normal erythrocyte

Plasmodium malariae Band form Trophozoite TROPHOZOITE STAGE

Plasmodium malariae SCHIZONT STAGE Rossete-like Merozoit (6-12) MEROZOITE Pigment

Plasmodium malariae Round/Oval Large nucleus, pale Reddish protoplasm GAMETOCYTE STAGE: Macro and Microgametocyte Round/Oval Small nucleus, compact Bluish protoplasm Round/Oval Large nucleus, pale Reddish protoplasm

Plasmodium falciparum TROPHOZOITE STAGE Fine ring Multiple infection

Plasmodium falciparum TROPHOZOITE STAGE Vacuole surrounded by plasma Maurer’s dots Vacuole

Plasmodium falciparum SCHIZONT STAGE Schizont occupy 2/3 erythrocyte 8-24 Merozoite

Plasmodium falciparum GAMETOCYTE STAGE: Macro and Microgametocyte Sausage shape Large nucleus, pale, more eccentric position Pigment dispersed Reddish protoplasm Banana/crescent shape Small nucleus, compact, in the middle Pigment around nucleus Bluish protoplasm

Plasmodium falciparum THICK BLOOD PREPARATION Plasmodium falciparum PLASMODIUM VIVAX Uniform No red zone around parasite Smaller than lymphocyte nucleus Plasmodium malariae Not uniform No red zone around parasite Smaller than lymphocyte nucleus Not uniform Red zone around parasite Bigger than lymphocyte nucleus