Milk Fat Globule Protein Epidermal Growth Factor-8

Slides:

Advertisements

Similar presentations

Tetrahydrocurcumin provides neuroprotection in rats after traumatic brain injury: autophagy and the PI3K/AKT pathways as a potential mechanism Yongyue.

Advertisements

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

ACE Inhibitor Quinapril Reduces the Arterial Expression of NF-κB-Dependent Proinflammatory Factors but not of Collagen I in a Rabbit Model of Atherosclerosis

Alpha-lipoic acid inhibits fractalkine expression and prevents neointimal hyperplasia after balloon injury in rat carotid artery Kyeong-Min Lee, Keun-Gyu.

Volume 14, Issue 4, Pages (May 2004)

Regulation of Neuronal Nitric Oxide Synthase in Rat Adrenal Medulla

Figure 2. Macrophages in dystrophic muscle in vivo and in vitro express Klotho. (A) A cross-section of 4-week-old mdx muscle labeled with antibodies to.

by Johannes B. K. Schwarz, Nicolas Langwieser, Nicole N

Vascular Endothelial–Cadherin Tyrosine Phosphorylation in Angiogenic and Quiescent Adult Tissues by Nathalie Lambeng, Yann Wallez, Christine Rampon, Francine.

Hypoxia-Inducible Factor-1α Mediates Increased Sympathoexcitation via Glutamatergic N-Methyl-d-Aspartate Receptors in the Paraventricular Nucleus of Rats.

Osteoprotegerin Disruption Attenuates HySu-Induced Pulmonary Hypertension Through Integrin αvβ3/FAK/AKT Pathway SuppressionCLINICAL PERSPECTIVE by Daile.

Intracellular Action of Matrix Metalloproteinase-2 Accounts for Acute Myocardial Ischemia and Reperfusion Injury by Wenjie Wang, Costas J. Schulze, Wilma.

Arterioscler Thromb Vasc Biol

Near Infrared Imaging and Photothermal Ablation of Vascular Inflammation Using Single-Walled Carbon Nanotubes by Hisanori Kosuge, Sarah P. Sherlock, Toshiro.

Sarcomere Mutation-Specific Expression Patterns in Human Hypertrophic CardiomyopathyClinical Perspective by Adam S. Helms, Frank M. Davis, David Coleman,

Parvalbumin Corrects Slowed Relaxation in Adult Cardiac Myocytes Expressing Hypertrophic Cardiomyopathy-Linked α-Tropomyosin Mutations by Pierre Coutu,

Molecular Imaging of Atherosclerotic Plaques Using a Human Antibody Against the Extra-Domain B of Fibronectin by Christian M. Matter, Pia K. Schuler, Patrizia.

Nitroxyl-Mediated Disulfide Bond Formation Between Cardiac Myofilament Cysteines Enhances Contractile FunctionNovelty and Significance by Wei Dong Gao,

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Volume 18, Issue 3, Pages (September 2013)

by Mineo Iwata, Lynn Graf, Norihiro Awaya, and Beverly Torok-Storb

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Volume 76, Issue 1, Pages (July 2009)

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Volume 80, Issue 6, Pages (September 2011)

A proteomic study of the aortic media in human thoracic aortic dissection: Implication for oxidative stress Mingfang Liao, MD, PhD, Zhaoyang Liu, PhD,

Experimental generation of a tissue-engineered functional and vascularized trachea Thorsten Walles, MD, Bettina Giere, Michael Hofmann, PhD, Johanna Schanz,

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Volume 69, Issue 8, Pages (April 2006)

Increased mTORC1 activity contributes to atherosclerosis in apolipoprotein E knockout mice and in vascular smooth muscle cells Kun Ling Ma, Jing Liu,

Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,

Molecular Therapy - Nucleic Acids

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

ADAMTS7 Cleavage and Vascular Smooth Muscle Cell Migration Is Affected by a Coronary-Artery-Disease-Associated Variant Xiangyuan Pu, Qingzhong Xiao,

Ascending aorta dilation in association with bicuspid aortic valve: A maturation defect of the aortic wall Nimrat Grewal, MD, Adriana C. Gittenberger-de.

Volume 129, Issue 3, Pages (September 2005)

Min Li, M. Sc. , Dong Liu, M. Sc. , Li Wang, Ph. D. , Weizhou Wang, B

International Journal of Cardiology

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Role for the endometrial epithelial protein MFG-E8 and its receptor integrin αvβ3 in human implantation: results of an in vitro trophoblast attachment.

Representative multiplex immunofluorescence image of synovial sarcoma with increased PD-L1/PD-1/CD8 cell densities at the tumor-invasive margin demonstrating.

Hui-Fang Song et al. BTS 2017;2:

Pingping Ren, MD, Lin Zhang, MS, Gaiping Xu, MD, Laura C

Volume 83, Issue 6, Pages (June 2013)

Comparative Proteomic Profiling of Murine Skin

TWEAK/Fn14 Signals Mediate Burn Wound Repair

(red) & endothelium (green) PMCA4 (green) & DAPI (blue)

Volume 16, Issue 2, Pages (July 2016)

Fig. 4. Identification of GRP78 as the target antigen of NMO-rAbs ON and ON Identification of GRP78 as the target antigen of NMO-rAbs.

Cluster analysis and pathway-based characterization of differentially expressed genes and proteins from integrated proteomics. Cluster analysis and pathway-based.

Yongji Wang, Megan L. Borchert, Hector F. DeLuca Kidney International

Min Li, M. Sc. , Dong Liu, M. Sc. , Li Wang, Ph. D. , Weizhou Wang, B

Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin Y. Luan, Ph.D., M.D., L. Kong, Ph.D.,

Aortic wall cell proliferation via basic fibroblast growth factor gene transfer limits progression of experimental abdominal aortic aneurysm Katsuyuki.

Molecular Therapy - Nucleic Acids

Volume 23, Issue 4, Pages (April 2015)

Volume 23, Issue 4, Pages (April 2015)

Proteomic Analysis of Mammalian Primary Cilia

Increased Expression of Wnt2 and SFRP4 in Tsk Mouse Skin: Role of Wnt Signaling in Altered Dermal Fibrillin Deposition and Systemic Sclerosis Julie Bayle,

Volume 82, Issue 3, Pages (August 2012)

YAP1 and IGF2BP3 regulate each other during fetal programming.

Morphologic changes induced in immortalized human podocytes after treatment with TGF-β1 (10 ng/mL, right) for 3 days when compared with control cells (left),

Artificial Zinc-Finger Transcription Factor of A20 Suppresses Restenosis in Sprague Dawley Rats after Carotid Injury via the PPARα Pathway Zhaoyou Meng,

S-Affs colocalize with SUMO in mammalian cells.

In vivo demonstration of EMT in GFP-tagged tumors.

The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3 pathway. TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3.

Aminoglycoside Enhances the Delivery of Antisense Morpholino Oligonucleotides In Vitro and in mdx Mice Mingxing Wang, Bo Wu, Sapana N. Shah, Peijuan.

Presentation transcript:

Milk Fat Globule Protein Epidermal Growth Factor-8 by Zongming Fu, Mingyi Wang, Marjan Gucek, Jing Zhang, James Wu, Liqun Jiang, Robert E. Monticone, Benjamin Khazan, Richard Telljohann, Julie Mattison, Simon Sheng, Robert N. Cole, Gaia Spinetti, Gianfranco Pintus, Lijuan Liu, Frank D. Kolodgie, Renu Virmani, Harold Spurgeon, Donald K. Ingram, Allen D. Everett, Edward G. Lakatta, and Jennifer E. Van Eyk Circulation Research Volume 104(12):1337-1346 June 19, 2009 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Quantification and characterization of MFG-E8 by 2DIDE and iTRAQ in aging rat aorta. Figure 1. Quantification and characterization of MFG-E8 by 2DIDE and iTRAQ in aging rat aorta. A, Enlarged region of 2D DIGE gel map showing that MFG-E8 is more abundant in aged aorta than young adult samples (2.53±0.15, n=8). Three solid white arrows point to gel spots identified as MFG-E8. Identifications were performed using light chromatography–MS/MS. B, iTRAQ MS spectra showing that MFG-E8 is more abundant in aged than young adult aorta. Representative MS spectra of 2 peptides VLPLSWHNR (409–417) and CLVTEDTQR (79–87) indicate the abundance of MFG-E8 is ≈2.5-fold increased. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. Validation and location of the MFG-E8 expression within the rat aortic wall. Figure 2. Validation and location of the MFG-E8 expression within the rat aortic wall. A, Average MFG-E8 transcriptome (n=4). B, 2DE Western blot of MFG-E8. C, Immunofluorescence staining for MFG-E8 (green) and α-SMA (red). Counterstaining nuclei with a DAPI dye (blue). Merged image shows staining overlap (yellow) (original magnification, ×200). D, Enlarged region of DIGE 2DE showing PNGase F–treated MFG-E8 compared to an untreated endogenous sample (red) from the old rat aorta. PNGase F treatment (green) results in the removal of N-linked carbohydrates, shifting the spot position to a lower molecular weight. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. The age-associated arterial MGF-E8 increase is conserved in mammalian species including humans. Figure 3. The age-associated arterial MGF-E8 increase is conserved in mammalian species including humans. A, Representative Western blots of monkey aortic MFG-E8 (left) and average data (right) (n=4). *P<0.05, old vs young. B, Immunohistostaining for MFG-E8 (brown color) within the monkey thoracic aortic wall (original magnification, ×100). L indicates lumen; M, media. C, Representative Western blots of human aortic MFG-E8 (left) and average data (right) (n=5). *P<0.05, old vs young. D, Double immunofluorescence staining for MFG-E8 (green) and α-SMA (red) and merged image (yellow) within the human thoracic aortic wall (original magnification, ×100). L indicates lumen; M, media. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Interplay between Ang II and MFG-E8 in vivo and in vitro. Figure 4. Interplay between Ang II and MFG-E8 in vivo and in vitro. A, Immunofluorescence staining for MFG-E8 (green) and Ang II (red) and merged image (yellow) in rat aortic wall. Nuclei counterstaining with DAPI (blue) (original magnification, ×400). L indicates lumen; M, media. B, Representative 1DE Western blots of MFG-E8 in smooth muscle cells isolated from young or old rat aorta with or without Ang II treatment with indicated concentration levels (upper images). The MFG-E8 immunoreactivity doublet is the N-linked glycosylated form (upper bands) and unglycosylated (lower bands), as shown by deglycosylation of the sample with PNGase F resulting in enhancement of the lower molecular weight band. Average data (lower graph). *P<0.05 old untreated and young treated cells vs young control cells. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Interaction between MFG-E8 and MCP-1 in vivo and in vitro. Figure 5. Interaction between MFG-E8 and MCP-1 in vivo and in vitro. A, Immunofluorescence staining for MFG-E8 (green) and MCP-1 (red) and merged image (yellow) in old human aorta. Nuclei counterstaining with DAPI (blue) (original magnification, ×200). L indicates lumen; M, media. B, Immunofluorescence photomicrographs (×400) for MFG-E8 (red color), MCP-1 (green color), nuclei (blue color), and colocalization of MFG-E8 and MCP-1 (yellow color, right images) within VSMCs. C, Representative Western blots show that the functional dimer of MCP-1 increases in VSMC treated with MFG-E8 in both young and old cells in a dose-dependent manner. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.

Figure 6. VSMC invasion assay. Figure 6. VSMC invasion assay. A, Average invasion analysis of VSMCs with and without siRNA of MFG-E8 (left graph). *P<0.05, old vs young cells chemoattracted by PDGF (10 ng/mL); #P<0.05, young and old cells with MFG-E8 silencing vs sham silencing, respectively. Representative Western blots show that the majority of MFG-E8 protein is knocked down via siRNA approach (right). B, Average invasion analysis of VSMCs with and without siRNA of MFG-E8 and Ang II treatment. *P<0.05, old vs young cells with treated with Ang II (100 nmol) and chemoattracted by PDGF (10 ng/mL); #P<0.05, young and old cells with MFG-E8 silencing vs sham silencing, respectively. C, Average invasion analysis of VSMCs treated with and without MFG-E8 plus vCCI. *P<0.05 young and old cells treated with MFG-E8 (50 ng/mL) vs untreated controls, respectively; #P<0.05, young and old cells treated with MFG-E8 plus vCCI vs MFG-E8 treated, respectively. Zongming Fu et al. Circ Res. 2009;104:1337-1346 Copyright © American Heart Association, Inc. All rights reserved.