DNA Technology.

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Presentation transcript:

DNA Technology

What is DNA technology? Analyzing DNA Cloning DNA Making Genetically Modified Organisms Forensic Sciences

Restriction Enzymes: Restriction Enzymes - The computer may say this is an unsecure site – its fine, just proceed. Introducing a Gene into a Cell Mechanism Of Recombination Restriction Endonucleases 1) What is a restriction enzyme/endonuclease and what does it do to DNA? 2) What is a “sticky end”? What is a “sticky end”? How are these used to make recombinant DNA? Define vectors and plasmids in your answer.

DNA Analysis How does DNA fingerprinting work? 3) What is a VNTR? How are they useful for DNA fingerprinting? 4) What is a STR? 5) What is PCR? Why is it used? 6) What is agarose? 7) How does gel electrophoresis work? 8) Why does DNA move toward the positive end of the electrophoresis chamber? Genetics of Taste – watch to 15:00

DNA Amplification: PCR Polymerase Chain Reaction PCR – step by step 9) What are the steps to PCR? 10) What is PCR? Why is it done? 11) How many copies of DNA are generated after 30 cycles of PCR?

Forensics Create A DNA Fingerprint 12) Who is the guilty party? How can you tell?

Cloning What is Cloning? Got Clones? 13) What is Cloning? 14) How does cloning differ from the formation of an identical twin? 15) What is Somatic Cell Nuclear Transfer? (Make sure you watch the video) 16) How is SCNT used to make clones? 17) Is cloning an organism the same as cloning a gene? Explain.

Viral Vectors NY Times Article (yes, you have to read) 18) How can viruses be used in the fight against cancer? Gene Therapy Explained 19) How does Gene Therapy work?

20) What is Genetic Engineering? 21) How does CRISPR work? Genetic Engineering Will Change Everything Forever 20) What is Genetic Engineering? 21) How does CRISPR work? 22) Opinion: Should genetic engineering of humans be done?

Genetically Modifies Organisms Why are GMOs bad? Genetically Modified Foods 22) Give three pros and three cons for GMOs. Transgenic Plants 23) How are transgenic plants created? What are the steps? Golden Rice Debate: 24) What is Golden Rice? 25) What are the two sides of the debate? Spider Goat: 26) How can spider silk be made by goats?