Published in Thyroid. January 2014, 24(1): DOI: /thy

Slides:

Advertisements

Similar presentations

Gross Techniques In Surgical Pathology. Introduction The routine work associated with a surgical pathology specimen includes gross & microscopic examinations.

Advertisements

PATHOPHYSIOLOGY PATHOPHYSIOLOGY w DEFINED Involves the study of function that results from disease processes.

Types of microscopes & Microtechniques.

Staining HAEMATOXYLIN & EOSIN

Histological Stains. Tissue Processing To some extent, light-level (as opposed to e.m.- level) histology is a lost art, especially if one is interested.

 Histology  Tissues - animal, human, plant  Sources - medical, veterinary, research  Grossed, fixed, processed, embedded, sectioned, and stained 

Introduction to Histology Tissue processing and Microscope

Histology and Embryology 组织学与胚胎学 Department of Histology and embryology Three Gorges University, Yichang, China.

Histology Histology is the study of the tissues of the body and how these tissues are arranged to constitute organs Literally, histology means tissue or.

Tissue Processing Dr : Hala El-sayed Mahmoud

PREPARATION OF HISTOLOGICAL SPECIMENS

The Process Of Molecular Cytology: Embedding and Sectioning Natasha Williams Dr. Katia Manova Zuckerman Research Building/ MSKCC.

TISSUE PREPARATION.

H&E STAINING Lab Lec # 4.

Course Descriptive Histology Introduction.

Histology Lab 1 Introduction and Epithelium Jun Zhou （周俊）, Ph.D & M.D School of Medicine,Zhejiang University.

Preparation of tissues for study

Professor KI-JONG RHEE Teaching assistant MIN HO LEE.

Introduction to Muscle Tissue. Skeletal Muscle - red fibres - white fibres - intermediate fibres Cardiac Muscle - cardiomyocytes - Purkinje fibres Smooth.

Pathophysiology.

Histological techniques: Haematoxylin and Eosin staining

Histology introduction

Practical Hematology Lab

Histological Techniques

Pathophysiology.

بسم الله الرحمن الرحيم Department of Pathology College of Medicine

In Search of the Optimal Set of Indicators when Classifying Histopathological Images Catalin Stoean University of Craiova, Romania

PATHOPHYSIOLOGY.

Neal et al.Supplementary Figure S1

Staining Methods.

Topic 1: Introduction to Histology

Figure S1 a b c d DAPI Lgr β-catenin Merge

General Principles of Tissue Preparation and Staining

Pathophysiology.

Volume 118, Issue 2, Pages (February 2000)

Published in Thyroid. January 2014, 24(1): DOI: /thy

Enhancing techniques to evaluate tumor margins

Fig. 1 Localized treatment of TNBC cancers kills tumor cells and minimizes the metastatic burden. Localized treatment of TNBC cancers kills tumor cells.

Standard step sectioning of skin biopsy specimens diagnosed as superficial basal cell carcinoma frequently yields deeper and more aggressive subtypes

Ryan S. Hennessy et al. BTS 2017;2:71-84

Nuclear c-Myc: A Molecular Marker for Early Stage Pemphigus Vulgaris

Figure 2 Histochemical and immunohistochemical staining and electron microscopic examination of structures in the brain biopsy Hematoxylin & eosin staining.

Published in Thyroid. January 2014, 24(1): DOI: /thy

Fig. 5 EGFR mutation status of patients with NSCLC, detected by histological examination and ARMS PCR. EGFR mutation status of patients with NSCLC, detected.

The Annals of Thoracic Surgery

Volume 3, Issue 1, Pages (January 2017)

Absence of Senescence-Associated β-Galactosidase Activity in Human Melanocytic Nevi In Vivo Murray A. Cotter, Scott R. Florell, Sancy A. Leachman, Douglas.

Interpretation of Histological sections

Paclitaxel Encapsulated in Cationic Liposomes Diminishes Tumor Angiogenesis and Melanoma Growth in a “Humanized” SCID Mouse Model Rainer Kunstfeld, Georg.

Fig. 4. Acute lung injury in miR-223−/y mice.

Elastic staining of paraffin-embedded lung tissue.

Published in Thyroid. January 2014, 24(1): DOI: /thy

H. Raffi, J.M. Bates, Z. Laszik, S. Kumar Kidney International

Herlina Y. Handoko, Neil F. Box, Graeme J. Walker

Volume 1, Issue 3, Pages (September 2007)

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

DDR1 plays an intrinsic role in primary epithelial cells undergoing branching morphogenesis and is required for correct organization of the basement membrane.

Rgs16::GFP expression in pancreatic neoplasia.

Expression of the Anti-Apoptotic Protein BAG3 in Human Melanomas

Knockout of SIRT1 in podocytes aggravates proteinuria and kidney injury in db/db mice. Knockout of SIRT1 in podocytes aggravates proteinuria and kidney.

Histology of Brucella bone marrow foci in mice.

Serial biopsy samples taken from the patient in figs 1and 2, showing from left to right: acute myocarditis, healing myocarditis, dilated cardiomyopathy.

Reversal of diabetic impairments in the blood flow recovery and neovascularization of ischemic areas by ANG-(1-7): A: Representative laser Doppler images.

A, TSG-6 staining in TRAMP tissue and TRAMP cell lines.

Tumors treated with anti-Flk-1 mAb have increased tumor cell apoptosis, reduced tumor cell proliferation, and increased tumor necrosis. s.c. Tumors treated.

Light microscopic evaluation of bladders and kidneys in E

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

a b c Supplementary Figure 1

(A) Gram stain of cyst fluid (magnification, ×200).

Presentation transcript:

Published in Thyroid. January 2014, 24(1): 88-168. DOI: 10. 1089/thy Published in Thyroid. January 2014, 24(1): 88-168. DOI: 10.1089/thy.2013.0109 © Mary Ann Liebert, Inc. FIG. 1. Microscopic structure of the mouse thyroid. (A) Hematoxylin and eosin (H&E) staining. (B) Periodic acid Schiff (PAS) staining. Mice were euthanized, and the thyroids dissected, fixed in buffered formalin, and embedded in paraffin. Thyroid sections (5 μm) were mounted on glass slides, de-paraffinated, and hydrated. For histological analysis, sections were stained with H&E, following a standard protocol. Glycoproteins were detected using PAS staining. Sections were stained with 0.5% periodic acid for 30 minutes and with Schiff's reagent for 20 minutes and then rinsed in running tap water for 5 minutes. Nuclei were counterstained with hematoxylin for 3 minutes. Sections were rinsed in running tap water, dehydrated, cleared, and mounted. Reproduced with permission from Senou et al. (20).