Chapter 9 Column Chromatography

Slides:

Advertisements

Similar presentations

Preparation of N,N-Diethyl-m-toluamide

Advertisements

When do we use Gravity Filtration? Filtration is the separation method of choice when the phases to be separated are a solid (precipitate) and a liquid.

Week 3 Collection and Analysis of Biodiesel

Chapter 8 Thin-Layer Chromatography

Extraction Lab # 6.

Analysis of Analgesic Tablets by Thin Layer Chromatography (TLC) Organic Chemistry Lab I Fall 2008 Dr. Milkevitch Oct 12 & 14, 2009.

Experiment 13 Molecular Weight Determination by Freezing Point Depression.

Today: Exp.6: Column Chromatography: The Separation of Ferrocene and Acetyl Ferrocene Midterm next Friday in your lab! starting at the beginning of your.

Experiment 15 A: Isolation of Pure Aspirin From Aspirin Tablets B: TLC of a Dye Mixture.

Column Chromatography. Types of columns: 1- Gravity Columns: The mobile phase move through the stationary phase by gravity force. 2- Flash Columns (Air.

What is Chromatography?

Distillation and Chromatography. Objectives Separate the components of a solution using distillation and chromatography Determine physical or chemical.

COPYRIGHT SAUTTER 2003 MOLE RELATIONSHIPS IN CHEMICAL REACTIONS (An Experimental Approach) WHAT IS A CHEMICAL REACTION? A PROCESS IN WHICH NEW SUBSTANCES.

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

PARTITION CHROMATOGRAPHY

Chromatography Lab # 5.

Physical Properties of Matter

Column Chromatography

Column and Thin Layer Chromatography

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

Experiment.13 Amino acid analysis by adsorption thin layer chromatography (adsorption TLC)

Chromatography Tomáš Mlčoch Pavel Moťka. Chromatography Described by Tswett in 1906 Described by Tswett in 1906 He separated some pigments using a tube.

Alexandria University Faculty Of Science Practical Training Course In Applications Of Plant Physiology Prepared By Dr. Mohamed Mohamed Ibrahim Lecturer.

Experimental Hypothesis The experimental hypothesis is that the process of steam distillation will yield a higher percent recovery of Eugenol from cloves.

COLUMN CHROMATOGRAPHIC PURIFICATION OF NITROANILINES

Experiment 5: COLUMN CHROMATOGRAPHIC PURIFICATION OF NITROANILINES.

Lecture 12 Chromatography Introduction Ch 7: Thin-Layer Chromatography Lecture Problem 4 Due This Week In Lab: Ch 6: Procedures 2 & 3 Due: Ch 5 Final Report.

Experiment 2 DISTILLATION AND GAS CHROMATOGRAPHY OF ALKANES.

COLUMN CHROMATOGRAPHIC PURIFICATION OF NITROANILINES

COLUMN CHROMATOGRAPHY (CC). TLC - Optimizing for column chromatography Optimum: 0.2 < R f < 0.5.

COLUMN CHROMATOGRAPHY

Separating Mixtures Chapter 25 Decanting Decanting means to pour off. Decanting is used in panning gold and in pouring water off vegetables.

By- Bhavya, Harsh, Harshvardhan, Namrata, Ronit and Vidhatri

Thin-Layer Chromatography (TLC) Uses: To separate the components of a mixture To determine the purity of a compound To see if two compounds are identical.

Chromatography A technique for separating and identifying components in a mixture. From the Greek: chroma = color and graph = writing. Involves the interaction.

S EPARATION BY CHROMATOGRAPHY Describe chromatography as an analytical technique that separates components in a mixture. State that the mobile phase may.

Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

Partition chromatography Partition chromatography is carried out on sheets of filter paper, column or thin layer of powdered cellulose, moist silica gel.

Gas Chromatography An Introduction. What is it? Gas chromatography – (gas-liquid chromatography) involves a sample being vaporized and injected onto the.

Separating Mixtures Must be a difference in physical properties to separate a mixture.

OXIDATION OF 9-FLUORENOL

T.A Nouf Alshareef KAU-Faculty of Science- Biochemistry department Analytical biochemistry lab (Bioc 343) 2012

Mixtures of Compounds & Elements Classification & Separation.

Chromatography.

Extraction Method #2 Fractionated precipitation: Dried plant of 6g is extracted with Methanol at a mass-volume ratio of 1:10 After extraction, the methanolic.

PAG6 TLC. Need to book Repro – Example results or Page 19 of delivery guide – Example method – FLIPPED learning sheet for start of nucleotides Applied.

CHROMATOGRAPHY.

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

Identification of Substances by Physical Properties.

Chromatography.

LU 3: Separation Technique (P2)

What is Chromatography?

Crystallization & Filtration

Separation Techniques

Making a solution. Dissolving

Biphenyl, Benzhydrol and Benzophenone

Separation Techniques

What is Chromatography?

SEPARATION TECHNIQUES

Separation of a Mixture

Acetylferrocene Ferrocene. Separating Ferrocene and Acetylferrocene by adsorption column chromatography.

ADSORPTION CHROMATOGRAPHY

What is Chromatography?

SEPARATION OF MIXTURE BY USING COLUMN CHROMATOGRAPHY

1/18 Objective: Explain the purpose and process of chromatography.

Physical Separation Techniques

What is Chromatography?

Presentation transcript:

Chapter 9 Column Chromatography

Column Chromatography (CC) Column chromatography is a useful method for separation and purification of solids and liquid samples under 10 grams. CC works on the same principle of TLC except that the sample solution flows down a column of silica gel or alumina filled with solvent. A partition coefficient represents the efficiency of the column and is determined by the solubility of the solute in the two phases.

Column Chromatography (CC) Only complete Part 1 (microscale) pp. 210-211, Ferrocene and Acetylferrocene. Packing the Column Make sure your column has a polyethylene frit. Pour the silica gel (or alumina) into the column so it is 1/2 to 2/3 full. Pour this silica gel into a 50 mL beaker and add 8 mL of hexane. Clamp the column in a vertical position. Add 2 mL of hexane to the column. Stir the hexane silica gel mixture while pouring into the column. Open the valve and drain the solvent. Do not let the solvent level get below the silica gel. Repeat the last three steps until all of the silica gel is in the column. Close the valve with some solvent remaining at the top of the column.

Column Chromatography (CC) Follow the handout for further experimental steps and obtain the following: Weight of crude ferrocene and of crude acetylferrocene. Melting points of ferrocene and of acetylferrocene. Percent Recovery

Comments Acetylferrocene is toxic. Handle with care. Hexane is flammable. Be aware of your surroundings. It should be easy to follow this separation process as both compounds are colored.