22 –Bacteriostatic Compounds, and Antibiotic Resistance

Slides:



Advertisements
Similar presentations
Kirby-Bauer disk diffusion Testing skill based learning
Advertisements

Antimicrobial Control Agents Mr. Shadi ALashi. Antimicrobial control agents Usually, microbial controls are used to avoid contamination of pure cultures,
ANTIBIOTIC By:Afnan Bakhsh. Sir Alexander Fleming (1881 –1955)  “One sometimes finds what one is not  looking for“ Penicillin He observed inhibition.
Bailey McCue Grade 9.  The purpose of this experiment was to find the weakest solution of bleach in water that would effectively inhibit the growth of.
Experiment 3 Culture of Microbe & Microbial Antibiotic Susceptibility Test.
Experiment two Cultivation Techniques of microorganism
Bailey McCue Grade 9 Academy of Notre Dame de Namur.
Experiment three Physical and Chemical agents for the Control of microorganisms Effect of Ultraviolet Radiation Effect of chemical agents.
Susceptibility of Antibacterial Chemicals on Pseudomonas aeruginosa Research performed by: Clayton Brady Health Science Biology Major Tennessee Technological.
STREAKING AN AGAR PLATE FOR ISOLATIONOF BACTERIAL COLONIES BACTERIAL COLONIES.
Screening for new antibiotics
Determination of MIC by Agar Diffusion Method. Minimum Inhibitory Concentration (MIC)  Definition: is the lowest concentration of an antimicrobial agent.
Antibiotics AMANY NIAZY
Antibiotic susceptibility testing Modified Kirby-Bauer method
Chemical Agents that Affect Microbial Growth.  A chemical substance used in treatment of infectious disease. ◦ Bacteriocidal agents.  Kill bacterial.
Ex. 12: Chemical Antimicrobial Agents: Antibiotics Objectives ??
Control of microbial growth. Antimicrobial Classes Disinfectants –Products aimed at reducing by at least five powers of 10 (99,999 %) the number of microorganisms/virus.
Chemotherapeutic and Chemical Agents Microbiology 156.
IN THE NAME OF ALLAH ALMIGHTY THE MOST COMPASSIONATE THE MERCIFUL.
Mic 224 Lab 6 Streak Plate Technique and Antibiotic Sensitivity.
PHT 226 Lab number 7 Total and viable count of bacteria.
Study the effect of industrial antibiotics on microbial growth.
Controlling Bacteria. Antimicrobial Agents (Bactericide) Chemical substances that either kill bacteria or inhibit bacterial growth without harming the.
PHT 416 Lab no 10 Minimum Inhibitory Concentration [MIC]
KIRBY – BAUER MINIMUM INHIBITORY CONCENTRATION MINIMUM BACTERIOCIDAL CONCENTRATION.
Lab 13 Microbial Control with Chemical Agents. Staphylococcus aureus Escherichia coli Do all disinfectants and antiseptics work equally well against.
Chapter 15: Antimicrobial Drugs ChemotherapyThe use of drugs to treat a disease Antimicrobial drugsInterfere with the growth of microbes within a host.
General Microbiology Laboratory Microbial control agents.
Pathogenic Microorganisms. Bacteria  Fungi  Parasites.
Lab 28 Goals and Objectives: Read results Exercise 31: Lethal Effect of UV Light Exercise 33: Effectiveness of Alcohol as an Antiseptic Exercise 36: Effectiveness.
Lab #10.
IN THE NAME OF ALLAH ALMIGHTY THE MOST COMPASSIONATE THE MERCIFUL.
Susceptibility (Sensitivity) Testing: Results Pharmaceutical Microbiology – Practical Course Semester One_ Sensitivity Results & MIC Broth Dilution/
Effects of physical, chemical and biological agents on bacteria Experiment 4.
Bailey McCue Grade 9.  The purpose of this experiment was to find the weakest solution of bleach in water that would effectively kill E. Coli and B.
Antibiotics Basmah almaarik
Antibiotic sensitivity
Microbiological Tests
Lab: 6 ANTIBIOTIC SENSITIVITY TESTING
"Don't forget to take a handful of our complimentary antibiotics on your way out“
Lab # 1. Antimicrobial Therapy  Natural antibiotic agents:  Produced by microorganisms:  Penicillium notatum – penicillin  Semi-synthetic antibiotic.
Effects of Radiation on Growth. Radiant energy released by certain electromagnetic processes Divided into a spectrum based on wavelength – Longer wavelength:
Ch 7 - Microbial Growth and Control
Chapter 42 Antimicrobial Sensitivity Testing
culturing Microorganisms
Antimicrobial Susceptibility Testing (AST)
Control of Microbial Growth
Que. 1 Which of the following methods will not sterilize?
ANTIBIOTIC SENSITIVITY
Effect of UV radiation on bacteria
Control of Pathogenic Bacteria
Exercise 33: Effectiveness of Alcohol as an Antiseptic
Chemical Antimicrobial Agents: Antibiotics
Antibiotic Sensitivity
Antibiotic Susceptibility Testing
**NO DISINFECTING, NO HAND WASHING before Ex.33**
Antibiogram By:Dr. S. S. Khoramrooz In the name of God
Antibiotics AMANY NIAZY
Agar diffusion method Basmah almaarik
Antibiotic Sensitivity
Antimicrobial Medications
Antibiotic sensitivity test
Antibiotic susceptibility testing
Streaking an Agar Plate For
Lab# 8 Kirby-Bauer test BCH462 [practical].
Antibiotics AMANY NIAZY
Antibiotics AMANY NIAZY
Bacteria and You! Helicobacter pylori Barry Marshal.
API 20 E System & Antibiotic susceptibility test
Presentation transcript:

22 –Bacteriostatic Compounds, and Antibiotic Resistance Dr. Souki Principles & Applications of Microbiology Cerritos College

The Inhibitory Action of Antiseptics and Disinfectants

Purpose To determine the effects of known disinfectants and antiseptics on bacterial growth.

FDA-Approved Antiseptics Tincture of Iodine 2% free iodine Povidone-iodine Betadine E-Z Scrub Silver sulfadiazine Creams, wound dressing

Common Disinfectants Bleach Ethanol or isopropanol 1:10 dilution household bleach 5250-6150 ppm hypochlorite ion Biocidal on most vegetative bacteria in seconds Inactivates most endospores in under 10 minutes Ethanol or isopropanol 70% Biocidal Does not inactivate endospores

Procedure Prepare nutrient agar plates Staphylococcus aureus Escherichia coli Mark quadrants on plate bottom Use sterile swab to inoculate Heat-sterilize forceps Dip disk in solution Solution 1:______ Solution 2: ______ Solution 3: ______ Solution 4: ______ Drop disk in center of quadrant Incubate RT, 48 hours Measure zone of inhibition

How is a zone of inhibition measured? Measure the diameter of the disk Measure the diameter of the zone of inhibition

Diameters of Zones of Inhibition (mm) Results Organism Diameters of Zones of Inhibition (mm) Solution 1 Solution 2 Solution 3 Solution 4 Staphylococcus aureus Escherichia coli

The Effects of UV Light on Bacterial Growth

Purpose To determine the effects of UV light on bacterial growth.

What is Ultraviolet Radiation UV penetrates cells and damages DNA

Procedure Work as a table. Obtain 1 NA plate. Prepare 1 plates of either Bacillus megaterium Escherichia coli Inoculate using sterile swab Expose half the plate, using index card, with lid open to UV light for prescribed amount time Incubate 37 C, 24 hours Count colonies

Results Table UV Exposure (sec) # Colonies 1 15 3 30 5 60 7 90 Table B. megaterium Table UV Exposure (sec) # Colonies 1 15 3 30 5 60 7 90 E. coli Table UV Exposure (sec) # Colonies 2 15 4 30 6 60 8 90

Detection of Antibiotic Resistance

Purpose To determine the if various bacterial cultures are antibiotic resistant, using the Kirby-Bauer disk-diffusion method.

Kirby-Bauer Disk Diffusion Method Antibiotic Bactericidal or bacteriostatic Penicillium (fungus) First source of penicillin Alexander Fleming Beta-lactam antibiotic Resistance due to beta-lactamases Inhibits cell wall synthesis in G+ & G-

Procedure Work in pairs. Obtain 2 NA plate. Prepare 2 plates Escherichia coli Bacillus megaterium Proteus vulgaris Staphylococcus aureus Pseudomonas aeruginosa Inoculate using sterile swab Apply antibiotic discs Incubate 37 C, 24 hours Measure zone of inhibition

Results Collect class data. P. vulgaris S. aureus E. coli B. megaterium P C Te S Data set 1 Data set 2 Ave Zone (mm) Susceptibility Key: P = Penicillin 10 ug C = Chloramphenicol 30 ug Te = Tetracycline 30 ug S = Streptomycin 10 ug Susceptibility: S = Susceptible I = Intermediate R = Resistant

Procedure Use the data you collected to determine if the bacteria are: Resistant Intermediate Susceptible