HTRF® cytokine assays

Cytokine Assays Applications: Cytokine quantification Readout of kinase activity or receptor activation Infectious disease Allergy studies Autoimmune disorders Streamlined protocols for cytokine measurement directly on cells or cell supernatant Sandwich immunoassay involving two monoclonal antibodies for batch to batch reproducibility Highly sensitive (< 15 pg/mL) Small sample size needed (down to 5µl) Adaptable to HTS No washing step and much lower sample volumes than traditional ELISA, making it both cost and time efficient HTRF biomarkers

A comprehensive line of ready-to-use kits for quantifying cytokines in cell supernatant or whole cell Comprehensive reagent supply: calibrator, conjugates, assay buffers and complete protocol Small and bulk size tests All batches systematically controlled Assays available for: (m&h) TNFa, (h) IL1b, (h) IL2, (h) IL4, (h) IL5, (m&h) IL6, (h) IL 8, (h)IL10, (m&h) IL17, (h) IL12, (m&h) IFNg, (h) GROa Looking for other cytokine assays ? Transform your ELISAs into a truly homogeneous high throughput method: Let us do it for you! HTRF biomarkers

Cytokine assays can be run using cell supernatants or whole cells Sandwich assays using a mix-and-measure procedure HTRF biomarkers

Cell-based and supernatant formats give similar results Not sure if we want the individual pages cytokines, PGE2 and Histamine to be more academic with bullets describing theory, etc. or if to leave more assay focused??? Slide text…. Cisbio offers kits for cytokine detection to address the screening of major inflammation targets HTRF biomarkers

Product specifications (1/3) (h) TNF alpha  (m) TNF alpha (h) IL1b  (h) IL2 *  (h)IL4 (h)IL5 Assay type: Sandwich  Sandwich Detection limit  10 pg/mL  5 pg/mL 15 pg/mL  39 pg/mL  16 pg/mL 39 pg/mL Working range Up to 6000 pg/mL Up to 10000 pg/mL Up to 5000 pg/mL Up to 10,000 pg/mL Incubation 3-24h at RT 3h at RT 2h at RT Diluent Phosphate buffer, culture medium * Assay on demand (frozen, available in 1,000 and 20,000 test volumes) HTRF biomarkers 6

Product specifications (2/3) (h) IL6  (m) IL6  (h) IL8 *  (h) IL10  (h) IL12  Assay type: Sandwich  Detection limit  15 pg/mL  40 pg/mL  15 pg/mL  10 pg/mL  30 pg/mL  Working range Up to 12500 pg/mL Up to 5000 pg/mL ND Incubation 3h at RT 2h at RT ON at 4C 4h at RT Diluent Phosphate buffer, culture medium * Assay on demand (frozen, available in 1,000 and 20,000 test volumes)

Product specifications (3/3) (h) IL17  (m) IL17* (h) IFNg (m) IFNg (h) GROa Assay type: Sandwich  Detection limit  56 pg/mL  10 pg/mL  30 pg/mL  19 pg/mL  Working range Up to 5000 pg/mL Up to 2000 pg/mL NA Incubation 24h at RT 2h at RT ON at RT 2h at 4°C Diluent Phosphate buffer, culture medium * Assay on demand (frozen, available in 1,000 and 20,000 test volumes)

Learn how they used the kits TNF-alpha Krinstin P et al., «  Two High Throughput Screen Assays for the Measurement of TNF-alpha in THP-1 Cells », Current chemical Genomics, 2011, 5, 21-29. IFN-gamma K Enamoto et al., «  High throughput screening for human interferon-gamma production inhibitor using homogenous time-resolved fluorescence. », J Biomol Screen. 2000 Aug;5(4):263-8 Cytokines S Achardet al., «  Homogeneous assays allow direct "in well" cytokine level quantification. », Assay Drug Dev Technol. 2003 Feb;1(1 Pt 2):181-5 HTRF biomarkers