MOLECULAR CELL BIOLOGY © 2008 W. H. Freeman and Company Lodish • Berk • Kaiser • Krieger • Scott • Bretscher •Ploegh • Matsudaira MOLECULAR CELL BIOLOGY SIXTH EDITION CHAPTER 13 Moving Proteins into Membranes and Organelles © 2008 W. H. Freeman and Company Copyright 2008 © W. H. Freeman and Company

Web Link: Protein Sorting

Part 1

Part 2

Web link: Synthesis of Secreted and Membrane Proteins

Web Links: Protein Translocation Binding immunoglobulin protein (BiP) BiP publication

Positive: Hydrophobic Negative: Polar

Common precursor of N-linked oligosaccharides 14 residue precursor Core Region (purple) five residues Uses Proper folding Stability Cell to Cell Adhesion (ABO groups)

Tunicamycin is mixture of homologous nucleoside antibiotics that inhibits the UDP-HexNAc: polyprenol-P HexNAc-1-P family of enzymes.

Cysteine PDI: Protein Disulfide Isomerase

Hemagglutinin folding and assembly

Influenza Virion HA proteins on surface

Protein import into the mitochondrial matrix Unfolded state Bound by chaperones Bind import receptor Import pore Moves through channel Removal of import sequence Active protein

Translocase of the Outer Mitochondrial membrane (TOM complex) Translocase of the Inner Mitochondrial membrane (TIM complex)

Peroxisome assembly protein (Pex)

PEX3

Ran-dependent