Hyperdiploid Acute Lymphoblastic Leukemia With 51 to 65 Chromosomes: A Distinct Biological Entity With a Marked Propensity to Undergo Apoptosis by Chikako.

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Hyperdiploid Acute Lymphoblastic Leukemia With 51 to 65 Chromosomes: A Distinct Biological Entity With a Marked Propensity to Undergo Apoptosis by Chikako Ito, Masa-aki Kumagai, Atsushi Manabe, Elaine Coustan-Smith, Susana C. Raimondi, Frederick G. Behm, K. Gopal Murti, Jeffrey E. Rubnitz, Ching-Hon Pui, and Dario Campana Blood Volume 93(1):315-320 January 1, 1999 ©1999 by American Society of Hematology

Percentage of cell recovery after 7 days of culture on stroma according to ploidy. Percentage of cell recovery after 7 days of culture on stroma according to ploidy. Numbers of leukemic lymphoblasts before and after culture were counted by flow cytometry as described in Materials and Methods. Horizontal bars indicate the median cell recovery in each ploidy group. The broken horizontal line indicates the overall median cell recovery. Chikako Ito et al. Blood 1999;93:315-320 ©1999 by American Society of Hematology

Percentage of cell recovery after 7 days of culture on stroma among different subgroups of hyperdiploid 51-to-65 ALL, including cases without and with duplications of chromosomes 4 and 10, ≤ or >55 chromosomes, and without and with structural chromosomal ab... Percentage of cell recovery after 7 days of culture on stroma among different subgroups of hyperdiploid 51-to-65 ALL, including cases without and with duplications of chromosomes 4 and 10, ≤ or >55 chromosomes, and without and with structural chromosomal abnormalities. Numbers of leukemic lymphoblasts before and after culture were counted by flow cytometry as described in Materials and Methods. Horizontal bars indicate the median cell recovery in each group. Chikako Ito et al. Blood 1999;93:315-320 ©1999 by American Society of Hematology

Kinetics of cell recovery during culture with and without stroma in one case of hyperdiploid ALL (55 chromosomes). Kinetics of cell recovery during culture with and without stroma in one case of hyperdiploid ALL (55 chromosomes). Numbers of leukemic lymphoblasts before and after culture were counted by flow cytometry as described in Materials and Methods. Each point indicate the means of two measurements. Chikako Ito et al. Blood 1999;93:315-320 ©1999 by American Society of Hematology

Hyperdiploid lymphoblasts undergo apoptosis despite the presence of stromal layers. Hyperdiploid lymphoblasts undergo apoptosis despite the presence of stromal layers. Flow cytometric dot plots represent assessment of apoptosis before (top panels) and after 2 days of culture (bottom panels) in one case of hyperdiploid ALL (55 chromosomes). Left panels illustrate FSC (a measurement of cell size) versus SSC (a measurement of cell granularity). Center panels illustrate binding of Annexin V FITC (an indicator of apoptosis) and staining with propidium iodide (PI; a sign of membrane permeability). Right panels illustrate the cells’ DNA content, measured by staining with PI after cell membrane permeabilization, and labeling with dUTP-BrdU, which is incorporated into cells with fragmented DNA. In all panels, events outside the gates correspond to cells at various stages of apopotic death. Chikako Ito et al. Blood 1999;93:315-320 ©1999 by American Society of Hematology

Hyperdiploid lymphoblasts adhere to stroma. Hyperdiploid lymphoblasts adhere to stroma. Scanning electron microscopy of hyperdiploid lymphoblasts after 3 days of culture on bone marrow–derived stroma. Most cells are dying despite tight adhesion to stromal elements. Chikako Ito et al. Blood 1999;93:315-320 ©1999 by American Society of Hematology