Chapter 6 A Tour of a Cell AP minnkow The differences between prokaryotes and eukaryotes The structure and function of organelles common to plants and animal cells The structure and function of organelles found only in plants cells or only in animal cells

Cell Theory: Overview All organisms are made of cells The cell is the simplest collection of matter that can live Cell structure is correlated to cellular function All cells are related by their descent from earlier cells

Protists, fungi, animals, and plants all consist of eukaryotic cells Concept 6.2: Eukaryotic cells have internal membranes that compartmentalize their functions The basic structural and functional unit of every organism is one of two types of cells: prokaryotic or eukaryotic Only organisms of the domains Bacteria and Archaea consist of prokaryotic cells Protists, fungi, animals, and plants all consist of eukaryotic cells

Characteristic Prokaryotic Cells Present (yes/no) Eukaryotic Cells Plasma membrane Cytosol with organelles Ribosomes Nucleus Size 1µm-10µm 10µm-100µm Internal Membranes

Prokaryotes What are 3 Key Features? Fimbriae Nucleoid Ribosomes Plasma membrane Bacterial chromosome Cell wall Capsule 0.5 µm Flagella (a) A typical rod-shaped bacterium (b) A thin section through the bacterium Bacillus coagulans (TEM)

Eukaryotes What are 3 Key Features?

TEM of a plasma membrane (a) Outside of cell Inside of cell 0.1 µm Carbohydrate side chain Hydrophilic region Hydrophobic region Hydrophilic region Phospholipid Proteins (b) Structure of the plasma membrane

Nucleus & Ribosomes

Concept 6.3: The eukaryotic cell’s genetic instructions are housed in the nucleus and carried out by the ribosomes The nucleus contains most of the DNA in a eukaryotic cell Nuclear Membrane Nuclear Pores Nuclear Lamina Chromatin Chromosomes Ribosomes use the information from the DNA to make proteins Consists of ribosomal RNA and Protein Bound Ribosomes (ER, Nuclear Env) Free Ribosomes

Close-up of nuclear envelope Fig. 6-10 Nucleus 1 µm Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Nuclear pore Pore complex Rough ER Surface of nuclear envelope Ribosome 1 µm 0.25 µm Close-up of nuclear envelope Pore complexes (TEM) Nuclear lamina (TEM)

Endoplasmic reticulum (ER) Fig. 6-11 Cytosol Endoplasmic reticulum (ER) Free ribosomes Bound ribosomes Large subunit Figure 6.11 Ribosomes Small subunit 0.5 µm TEM showing ER and ribosomes Diagram of a ribosome

Endoplasmic Reticulum Golgi Apparatus Vescicles Lysosomes

Components of the endomembrane system: Concept 6.4: The endomembrane system regulates protein traffic and performs metabolic functions in the cell Components of the endomembrane system: Nuclear envelope Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Plasma membrane These components are either continuous or connected via transfer by vesicles

Nucleus Rough ER Smooth ER Plasma membrane Fig. 6-16-1 Nucleus Rough ER Smooth ER Figure 6.16 Review: relationships among organelles of the endomembrane system Plasma membrane

The ER membrane is continuous with the nuclear envelope The endoplasmic reticulum (ER) accounts for more than half of the total membrane in many eukaryotic cells The ER membrane is continuous with the nuclear envelope There are two distinct regions of ER: Smooth ER, which lacks ribosomes Rough ER, with ribosomes studding its surface The smooth ER Synthesizes lipids Metabolizes carbohydrates Detoxifies poison Stores calcium The rough ER Has bound ribosomes, which secrete glycoproteins (proteins covalently bonded to carbohydrates) Distributes transport vesicles, proteins surrounded by membranes Is a membrane factory for the cell

Nucleus Rough ER Smooth ER cis Golgi Plasma membrane trans Golgi Fig. 6-16-2 Nucleus Rough ER Smooth ER cis Golgi Figure 6.16 Review: relationships among organelles of the endomembrane system Plasma membrane trans Golgi

Functions of the Golgi apparatus: The Golgi apparatus consists of flattened membranous sacs called cisternae Functions of the Golgi apparatus: Modifies products of the ER Manufactures certain macromolecules Sorts and packages materials into transport vesicles

Nucleus Rough ER Smooth ER cis Golgi Plasma membrane trans Golgi Fig. 6-16-3 Nucleus Rough ER Smooth ER cis Golgi Figure 6.16 Review: relationships among organelles of the endomembrane system Plasma membrane trans Golgi

A lysosome is a membranous sac of hydrolytic enzymes that can digest macromolecules Lysosomal enzymes can hydrolyze proteins, fats, polysaccharides, and nucleic acids

Endocytosis/Exocytosis Some types of cell can engulf another cell by phagocytosis; this forms a food vacuole pinocytosis; exocytosis A lysosome fuses with the food vacuole and digests the molecules Lysosomes also use enzymes to recycle the cell’s own organelles and macromolecules, a process called autophagy

Nucleus 1 µm Lysosome Digestive enzymes Lysosome Plasma membrane Fig. 6-14a Nucleus 1 µm Lysosome Digestive enzymes Lysosome Figure 6.14a Lysosome—phagocytosis Plasma membrane Digestion Food vacuole (a) Phagocytosis

two damaged organelles 1 µm Fig. 6-14b Vesicle containing two damaged organelles 1 µm Mitochondrion fragment Peroxisome fragment Lysosome Figure 6.14b Lysosomes—autophagy Peroxisome Mitochondrion Digestion Vesicle (b) Autophagy

A plant cell or fungal cell may have one or several vacuoles Food vacuoles are formed by phagocytosis Contractile vacuoles, found in many freshwater protists, pump excess water out of cells Central vacuoles, found in many mature plant cells, hold organic compounds and water

Central vacuole Cytosol Nucleus Central vacuole Cell wall Chloroplast Fig. 6-15 Central vacuole Cytosol Figure 6.15 The plant cell vacuole Nucleus Central vacuole Cell wall Chloroplast 5 µm

Concept 6.5: Mitochondria and chloroplasts change energy from one form to another Mitochondria are the sites of cellular respiration, a metabolic process that generates ATP Chloroplasts, found in plants and algae, are the sites of photosynthesis Peroxisomes are oxidative organelles Mitochondria and chloroplasts Are not part of the endomembrane system Have a double membrane Have proteins made by free ribosomes Contain their own DNA For the Cell Biology Video ER and Mitochondria in Leaf Cells, go to Animation and Video Files. For the Cell Biology Video Mitochondria in 3D, go to Animation and Video Files. For the Cell Biology Video Chloroplast Movement, go to Animation and Video Files.

Mitochondria

in the mitochondrial matrix Fig. 6-17 Intermembrane space Outer membrane Free ribosomes in the mitochondrial matrix Inner membrane Cristae Figure 6.17 The mitochondrion, site of cellular respiration Matrix 0.1 µm

Mitochondria: Chemical Energy Conversion Mitochondria are in nearly all eukaryotic cells They have a smooth outer membrane and an inner membrane folded into cristae The inner membrane creates two compartments: intermembrane space and mitochondrial matrix Some metabolic steps of cellular respiration are catalyzed in the mitochondrial matrix Cristae present a large surface area for enzymes that synthesize ATP

Chloroplasts

Chloroplast structure includes: Thylakoids, membranous sacs, stacked to form a granum Stroma, the internal fluid Ribosomes Stroma Inner and outer membranes Granum 1 µm Thylakoid

Chloroplasts: Capture of Light Energy The chloroplast is a member of a family of organelles called plastids Chloroplasts contain pigment (chlorophyll and others) as well as enzymes and other molecules that function in photosynthesis Chloroplasts are found in leaves and other green organs of plants and in algae

Peroxisomes

Chloroplast Peroxisome Mitochondrion 1 µm Figure 6.19 A peroxisome

Peroxisomes: Oxidation Peroxisomes are specialized metabolic compartments bounded by a single membrane Peroxisomes produce hydrogen peroxide and convert it to water Oxygen is used to break down different types of molecules such as alcohols and lipids Transfers H from molecules to O

Cytoskeleton Centrioles Cilia Flagella

It is composed of three types of molecular structures: Concept 6.6: The cytoskeleton is a network of fibers that organizes structures and activities in the cell The cytoskeleton is a network of fibers extending throughout the cytoplasm It organizes the cell’s structures and activities, anchoring many organelles It is composed of three types of molecular structures: Microtubules Microfilaments Intermediate filaments For the Cell Biology Video The Cytoskeleton in a Neuron Growth Cone, go to Animation and Video Files For the Cell Biology Video Cytoskeletal Protein Dynamics, go to Animation and Video Files.

10 µm Column of tubulin dimers Tubulin dimer   25 nm Table 6-1a

Table 6-1b 10 µm Table 6-1b Actin subunit 7 nm

Fibrous subunit (keratins coiled together) Table 6-1c 5 µm Table 6-1c Keratin proteins Fibrous subunit (keratins coiled together) 8–12 nm

Microtubule Microfilaments 0.25 µm Figure 6.20 The cytoskeleton

Roles of the Cytoskeleton: Support, Motility, and Regulation The cytoskeleton helps to support the cell and maintain its shape It interacts with motor proteins to produce motility Inside the cell, vesicles can travel along “monorails” provided by the cytoskeleton Recent evidence suggests that the cytoskeleton may help regulate biochemical activities

Receptor for motor protein Fig. 6-21 Vesicle ATP Receptor for motor protein Motor protein (ATP powered) Microtubule of cytoskeleton (a) Microtubule Vesicles 0.25 µm Figure 6.21 Motor proteins and the cytoskeleton (b)

Centrosomes & Centrioles

Centrosomes and Centrioles In many cells, microtubules grow out from a centrosome near the nucleus The centrosome is a “microtubule-organizing center” In animal cells, the centrosome has a pair of centrioles, each with nine triplets of microtubules arranged in a ring

Longitudinal section of one centriole Microtubules Cross section Fig. 6-22 Centrosome Microtubule Centrioles 0.25 µm Figure 6.22 Centrosome containing a pair of centrioles Longitudinal section of one centriole Microtubules Cross section of the other centriole

Cilia & Flagella

Cilia and Flagella Microtubules control the beating of cilia and flagella, locomotor appendages of some cells Cilia and flagella differ in their beating patterns

Direction of organism’s movement Fig. 6-23 Direction of swimming (a) Motion of flagella 5 µm Direction of organism’s movement Figure 6.23a A comparison of the beating of flagella and cilia—motion of flagella Power stroke Recovery stroke (b) Motion of cilia 15 µm

Cilia and flagella share a common ultrastructure: A core of microtubules sheathed by the plasma membrane A basal body that anchors the cilium or flagellum A motor protein called dynein, which drives the bending movements of a cilium or flagellum

Cross section of cilium Fig. 6-24 Outer microtubule doublet Plasma membrane 0.1 µm Dynein proteins Central microtubule Radial spoke Protein cross-linking outer doublets Microtubules (b) Cross section of cilium Plasma membrane Basal body 0.5 µm (a) Longitudinal section of cilium 0.1 µm Figure 6.24 Ultrastructure of a eukaryotic flagellum or motile cilium Triplet (c) Cross section of basal body

Moving Cilia and Flagella How dynein “walking” moves flagella and cilia: Dynein arms alternately grab, move, and release the outer microtubules Protein cross-links limit sliding Forces exerted by dynein arms cause doublets to curve, bending the cilium or flagellum For the Cell Biology Video Motion of Isolated Flagellum, go to Animation and Video Files. For the Cell Biology Video Flagellum Movement in Swimming Sperm, go to Animation and Video Files.

Cross-linking proteins inside outer doublets Fig. 6-25b ATP Cross-linking proteins inside outer doublets Anchorage in cell (b) Effect of cross-linking proteins Figure 6.25b, c How dynein “walking” moves flagella and cilia 1 3 2 (c) Wavelike motion

Microfilaments (Actin Filaments) Microfilaments are solid rods about 7 nm in diameter, built as a twisted double chain of actin subunits The structural role of microfilaments is to bear tension, resisting pulling forces within the cell They form a 3-D network called the cortex just inside the plasma membrane to help support the cell’s shape Bundles of microfilaments make up the core of microvilli of intestinal cells

Microfilaments (actin filaments) Fig. 6-26 Microvillus Plasma membrane Microfilaments (actin filaments) Figure 6.26 A structural role of microfilaments Intermediate filaments 0.25 µm

Fig, 6-27a Microfilaments that function in cellular motility contain the protein myosin in addition to actin Muscle cell Actin filament Myosin filament Myosin arm Figure 6.27a Microfilaments and motility (a) Myosin motors in muscle cell contraction

Cortex (outer cytoplasm): gel with actin network The same action in muscles helps with ameboid movements and cytoplasmic streaming Inner cytoplasm: sol with actin subunits Extending pseudopodium (b) Amoeboid movement Nonmoving cortical cytoplasm (gel) Chloroplast Streaming cytoplasm (sol) Vacuole Parallel actin filaments Cell wall (c) Cytoplasmic streaming in plant cells

Cortex (outer cytoplasm): gel with actin network Fig. 6-27bc Cortex (outer cytoplasm): gel with actin network Inner cytoplasm: sol with actin subunits Extending pseudopodium (b) Amoeboid movement Nonmoving cortical cytoplasm (gel) Chloroplast Streaming cytoplasm (sol) Figure 6.27b,c Microfilaments and motility Vacuole Parallel actin filaments Cell wall (c) Cytoplasmic streaming in plant cells

Cytoplasmic streaming is a circular flow of cytoplasm within cells This streaming speeds distribution of materials within the cell In plant cells, actin-myosin interactions and sol-gel transformations drive cytoplasmic streaming Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

These extracellular structures include: Concept 6.7: Extracellular components and connections between cells help coordinate cellular activities Most cells synthesize and secrete materials that are external to the plasma membrane These extracellular structures include: Cell walls of plants The extracellular matrix (ECM) of animal cells Intercellular junctions For the Cell Biology Video Ciliary Motion, go to Animation and Video Files. Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

Cell Wall The cell wall is an extracellular structure that distinguishes plant cells from animal cells Prokaryotes, fungi, and some protists also have cell walls

Plant cell walls may have multiple layers: Primary cell wall: relatively thin and flexible Middle lamella: thin layer between primary walls of adjacent cells Secondary cell wall (in some cells): added between the plasma membrane and the primary cell wall Plasmodesmata are channels between adjacent plant cells

Secondary cell wall Primary cell wall Middle lamella Central vacuole Fig. 6-28 Secondary cell wall Primary cell wall Middle lamella 1 µm Central vacuole Cytosol Figure 6.28 Plant cell walls Plasma membrane Plant cell walls Plasmodesmata

Extracellular Matrix Functions of the ECM: Support Adhesion Movement Regulation

The Extracellular Matrix (ECM) of Animal Cells Animal cells lack cell walls but are covered by an elaborate extracellular matrix (ECM) The ECM is made up of glycoproteins such as collagen, proteoglycans, and fibronectin ECM proteins bind to receptor proteins in the plasma membrane called integrins For the Cell Biology Video Cartoon Model of a Collagen Triple Helix, go to Animation and Video Files. For the Cell Biology Video Staining of the Extracellular Matrix, go to Animation and Video Files. For the Cell Biology Video Fibronectin Fibrils, go to Animation and Video Files.

Proteoglycan complex Collagen EXTRACELLULAR FLUID Fibronectin Fig. 6-30a Collagen Proteoglycan complex EXTRACELLULAR FLUID Fibronectin Integrins Plasma membrane Figure 6.30 Extracellular matrix (ECM) of an animal cell, part 1 Micro-filaments CYTOPLASM

Polysaccharide molecule Fig. 6-30b Polysaccharide molecule Carbo-hydrates Core protein Figure 6.30 Extracellular matrix (ECM) of an animal cell, part 2 Proteoglycan molecule Proteoglycan complex

Intercellular Junctions Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact There are several types of intercellular junctions Plasmodesmata Tight junctions Desmosomes Gap junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

Plasmodesmata in Plant Cells Plasmodesmata are channels that perforate plant cell walls Through plasmodesmata, water and small solutes (and sometimes proteins and RNA) can pass from cell to cell Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

Cell walls Interior of cell Interior of cell 0.5 µm Plasmodesmata Fig. 6-31 Cell walls Interior of cell Interior of cell Figure 6.31 Plasmodesmata between plant cells 0.5 µm Plasmodesmata Plasma membranes

Tight Junctions, Desmosomes, and Gap Junctions in Animal Cells At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid Desmosomes (anchoring junctions) fasten cells together into strong sheets Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

Microscopy Scientists use microscopes to visualize cells too small to see with the naked eye In a light microscope (LM), visible light passes through a specimen and then through glass lenses, which magnify the image Magnification – the ratio of an object’s image size to its real size Resolution – the measure of the clarity of the image, or the minimum distance of two distinguishable points Contrast – visible differences in parts of the sample

Microscopy Two basic types of electron microscopes (EMs) are used to study subcellular structures Scanning electron microscopes (SEMs) – focus a beam of electrons onto the surface of a specimen, providing images that look 3-D Transmission electron microscopes (TEMs) – focus a beam of electrons through a specimen TEMs are used mainly to study the internal structure of cells