Neurogenesis Neural stem cells (NSC) were extracted from the subgranular zone of the dentate gyrus of the hippocampus of adult mice. NSC were induced.

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Neurogenesis Neural stem cells (NSC) were extracted from the subgranular zone of the dentate gyrus of the hippocampus of adult mice. NSC were induced to proliferate → “Neurosphere” formed Addition of Test compounds: Saline / Harmine / Tetrahydroharmine (0.5µM). Assessment of capacity of compounds to promote: Early stages of Neurogenesis (young neurons): TuJ1 staining (green) Neuronal Maturation (mature neurons): MAP-2 staining (red)

Distal region of Neurosphere No effects after saline Color code Blue staining: cell nuclei (marks all cells) Green staining: young neurons Red staining: mature neurons

Harmine Stimulates Neurogenesis in vitro… Color code Blue staining: cell nuclei (marks all cells) Green staining: young neurons Red staining: mature neurons

…and so does THH Color code Blue staining: cell nuclei (marks all cells) Green staining: young neurons Red staining: mature neurons