Screening of sperm velocity by fluid mechanical characteristics of a cyclo-olefin polymer microfluidic sperm-sorting device Koji Matsuura, Mami Takenami,

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Screening of sperm velocity by fluid mechanical characteristics of a cyclo-olefin polymer microfluidic sperm-sorting device Koji Matsuura, Mami Takenami, Yuka Kuroda, Toru Hyakutake, Shinichiro Yanase, Keiji Naruse Reproductive BioMedicine Online Volume 24, Issue 1, Pages 109-115 (January 2012) DOI: 10.1016/j.rbmo.2011.09.005 Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A) Schematic presentation of the microfluidic sperm-sorting (MFSS) principle: an illustration of two laminar flows (A→C and B→D) in the MFSS channel. (B) A cyclo-olefin-MFSS chip used in this study. (C, D) Definition of the microfluidic channel parameters. Axes x, y and z are length, width and height, respectively. Top and side views are shown in C and D, respectively. (E) Reconstructed, fluorescent, cross-sectional images of the microfluidic channels in chip A (left) and chip B (right). Reproductive BioMedicine Online 2012 24, 109-115DOI: (10.1016/j.rbmo.2011.09.005) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Images of motile sperm tracking, (A) before microfluidic sperm-sorting (MFSS) treatment and (B) in reservoir D of the cyclo-olefin-MFSS microfluidic channel. (C) Linear velocity distributions analysed from the images. Yellow=spermatozoa with the indicated velocity range without cyclo-olefin MFSS treatment; red=spermatozoa with the indicated velocity distribution in reservoir D of chip A; blue=spermatozoa with the indicated velocity distribution in reservoir D of chip B. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2012 24, 109-115DOI: (10.1016/j.rbmo.2011.09.005) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 (A) Definition of the parameters vxobs, vyobs, ϕ, vs and vf. (B) The correlation of vxobs and vyobs from confocal (red triangles) and bright-field (black squares) microscopy. The value of vf is between the values of vxobs and vxobs−100 μm/s. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2012 24, 109-115DOI: (10.1016/j.rbmo.2011.09.005) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions