Enzymes Lab 5

Chemical reaction The cells carries out many chemical reactions. A + B C +D reactants products

Enzymatic action Enzymes are organic catalysts that speed metabolic reactions. Most of the enzymes are proteins. The reactants in an enzymatic chemical reaction are called substrates. E + S ES E + P

Enzymes can be used over and over again. Enzymes have specific shape accommodating the shapes of their substrate. Enzymes can be used over and over again. Active site

Enzyme's optimal conditions The rate of an enzymatic reaction depends on conditions in the immediate environment These conditions affect the shape of the enzyme and modify the active site and precise fit of an enzyme and its substrate Factors influencing enzyme functions include temperature, PH, concentration of substrate and enzyme.

Enzyme denature Any structural change in an enzyme may denature or destroy its effectiveness by altering the active site and slowing down the reaction. Denatured enzymes may result from extreme temperature, extreme PH, or any environmental condition that fundamentally alters a protein's structure.

Goals of today's experiment To examine enzyme specificity To determine the optimal temperature for sucrosase To determine the optimal PH for sucrosae To compare enzyme performances under different concentrations

Following handout