Increased levels of ATRi-induced replication stress in AMLMLL cells.

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Increased levels of ATRi-induced replication stress in AMLMLL cells. Increased levels of ATRi-induced replication stress in AMLMLL cells. (A) Western blot of CHK1, RPA, and γH2AX phosphorylation in AMLMLL cells exposed to HU (2 mM, 2 hours). Data are representative of two independent experiments. (B) FACS analysis showing the percentage of viable AMLMLL and AMLETO cells (identified by size and DAPI exclusion) either untreated or exposed to ATRi (3 μM, 16 hours). Data are representative of two independent experiments. (C) FACS analysis of DNA content (PI) and H2AX phosphorylation in AMLMLL and AMLETO cells exposed to ATRi (10 μM, 5 hours). Data are representative of two independent experiments. (D) Fork rates were measured in stretched DNA fibers prepared from AMLMLL and AMLETO cells exposed (or not) to ATRi (10 μM, 5 hours). At least 200 tracks were measured per condition. ***P < 0.001 by two-tailed t test. Isabel Morgado-Palacin et al., Sci. Signal. 2016;9:ra91 ©2016 by American Association for the Advancement of Science