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BIND  TRANSFORM  RELEASE PROTEIN PHYSICS LECTURE 24 PROTEINS AT ACTION: BIND  TRANSFORM  RELEASE

BIND: repressors - turn - 

Zn- fingers DNA & RNA BINDING Leu-zipper

BIND  TRANSFORM  BIND: Repressors -BINDING-INDUCED DEFORMATION MAKES REPRESSOR ACTIVE, and IT BINDS TO DNA

BIND: Immunoglobulins

Immunoglobulin

BIND  TRANSFORM  RELEASE: ENZYMES chymotrypsin Note small active site

Chymotrypsin catalyses hydrolysis of a peptide Spontaneous hydrolysis: very slow

chymotrypsin

CHAIN CUT-INDUCED DEFORMATION MAKES ENZYME ACTIVE non-active cat. site  active cat. site Chymotripsin Chymotripsinogen

SER-protease: catalysis

CHYMOTRYPSIN ACTIVE SITE with INHIBITOR

Preferential binding of TS: RIGID enzyme Catalytic antibodies ABZYM = AntyBody enZYM Transition state (TS) Preferential binding of TS: RIGID enzyme Antibodies are selected to TS-like molecule