Intracellular Trafficking

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Presentation transcript:

Intracellular Trafficking How proteins know where to go

Nucleus Nuclear import & Export

+

Mitochondria Import to the Mitochondrial Matrix & Intramembranous Space

- - - - - - - - + + + + + + + + - - - - - - - -

Endoplasmic Reticulum Sorting to the ER lumen & Insertion in the ER membrane

hydropathy plot H2N COOH

NH2 COOH H2N COOH NH2 COOH

hydropathy plot H2N COOH

Endoplasmic Reticulum Modification at the ER and Folding of Soluble Proteins

“Post-translational modifications” = co-translational glycosylation addition of lipids protein folding (removal of signal peptide)

“consensus sequence” = Asn-X-Ser/Thr N-linked glycosylation “consensus sequence” = Asn-X-Ser/Thr

Protein Folding

ER “Retention” KDEL = Lys-Asp-Glu-Leu-C term

Vesicular Transport Secretory Pathway

“constitutive secretory pathway”

COP = Coatomer Protein

Endocytosis Exocytosis

Figure 13-13b Molecular Biology of the Cell (© Garland Science 2008)

Sar 1 hydrolyzes GTP GDP Sar 1 is no longer in the vesicle membrane Sec proteins no longer coat the vesicle (i.e., coat disassembles) Figure 13-13d Molecular Biology of the Cell (© Garland Science 2008)

Rab Proteins SNARE Proteins

Figure 13-16 Molecular Biology of the Cell (© Garland Science 2008)

Figure 13-17 Molecular Biology of the Cell (© Garland Science 2008)

Page 779 Molecular Biology of the Cell (© Garland Science 2008)

Figure 13-43 Molecular Biology of the Cell (© Garland Science 2008) P

lysosome pH decrease Figure 13-44 Molecular Biology of the Cell (© Garland Science 2008)

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