Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments.

Slides:

Advertisements

Similar presentations

Gel Electrophoresis Lab Analysis of Precut Lambda DNA.

Advertisements

Biotech Lab #5 DNA Goes to the Races “Gel electrophoresis”

“Gel electrophoresis”. Gel electrophoresis is a procedure for separating a mixture of molecules through a stationary material (gel) in an electrical field.

Gel Electrophoresis & Gel Loading Practice. Gel Electrophoresis The process by which electricity is used to separate charged molecules (DNA fragments,

BIO-RAD Forensic DNA Fingerprinting Kit

Gel Electrophoresis.

4.4 Using Gel Electrophoresis to Study Gene Molecules

Gel Electrophoresis Biotech I.

Electrophoresis Electrophoresis is the movement of molecules by an electric current .This is practically done in a matrix to limit migration and contain.

Aim: How do scientists identify people using DNA Fingerprinting?

DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!

Unit 8 Vocabulary 1. micropipette- tool used to transfer small volumes of liquid 2. P 20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used.

Lab 23 Goals and Objectives: ***Begin lab before lecture!!! EDVOKIT#124: DNA-based Screening for Smallpox Practice loading samples into “submarine gels”

 DNA can be collected from any living tissue:  blood, skin, hair, urine, semen, sweat or tears.

Agarose (Horizontal) Gel Electrophoresis Malasian word for seaweed is “agar-agar”. Agarose is derived from red seaweed. Electrophoresis means “carrying.

PCR is DNA replication in a test tube Ex. 25: PCR Based Testing for Water Contaminants Day 2.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

Gel Electrophoresis.

Gel Electrophoresis. Definition – COPY ME! Separation of DNA fragments according to size and charge Based on movement through a gel medium when an.

DNA Fingerprinting Comparing DNA samples using gel electrophoresis.

BIO-RAD Lambda DNA Kit, and BIO-RAD Forensic DNA Fingerprinting Kit Section Concepts: DNA Scissors Activity, restriction enzymes, gel electrophoresis,

Agarose gel electrophoresis. Agarose gel electrophoresis is an easy way to separate DNA fragments by their sizes and visualize them. It is a common diagnostic.

Gel Electrophoresis of DNA. DNA as Forensic Evidence Individual evidence – identify a single person Trace evidence – small amount left at crime scene.

DNA fingerprinting is not taking someone’s fingerprint. It is cutting up a DNA strand and separating them by size.

Gel Electrophoresis.

Gel Electrophoresis of DNA. What is Gel Electrophoresis? Electro = flow of electricity, phoresis, from the Greek = to carry across A gel is a colloid,

What happens now that the DNA has been extracted?

LAB 6 DNA FINGERPRINTING. BUILD the GEL FRAME Position comb and lock in place in side slots.

DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

Gel Electrophoresis. What is Gel Electrophoresis? Electrophoresis: Movement of charged particles through a solution (gel) under the influence of an electric.

Aim: How do scientists identify people using DNA Fingerprinting?

Gel Electrophoresis.

Aim: How do scientists identify people using DNA Fingerprinting?

***Begin lab before lecture!!!

Molecular Biology Part II: Gel Electrophoresis

How do scientists identify people using DNA Fingerprinting?

Using Gel Electrophoresis to Study Molecules

List general characteristics of all races

By Zainab sajjad (117114) Ayesha Rehman (117115)

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

Aim: How do scientists identify people using DNA Fingerprinting?

Gel Electrophoresis Exhibit: home made agar gel electrophoresis

Aim: How do scientists identify people using DNA Fingerprinting?

Gel Electrophoresis of DNA

Inquiry into gel electrophoresis

DNA Fingerprinting.

Introduction to Gel Electrophoresis

CSI: Waukegan Need to Knows

Biotech Lab #3 DNA Goes to the Races

DNA ELECTROPHORESIS OR DNA FINGERPRINTING.

Sequencing and Copying DNA

DNA Technology: GEL ELECTROHPHORESIS

Electrophoresis.

DNA Technology: GEL ELECTROHPHORESIS

DNA Fingerprinting and Gel Electrophoresis Notes

Agarose Gel Electrophoresis

Date: January 31th, 2017 Aim #46: How do scientists identify people using DNA Fingerprinting? HW: Daily Review of Class Notes Biotechnology Textbook.

Restriction Enzymes The ability to cut and paste DNA predictably is due to the use of restriction enzymes. They were first identified in and isolated.

Forging the Innovation Generation

Creating a DNA Fingerprint by Gel Electrophoresis

Introduction to Gel Electrophoresis

Gel Electrophoresis Ms. Cuthrell.

Another way to separate mixtures!

DNA Agarose Gel Electrophoresis

How to fingerprint the bad guy

Gel Electrophoresis: Introduction and Techniques

Gel Electrophoresis Gel Electrophoresis.

Presentation transcript:

Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments

Terms: Gel: matrix used to separate DNA usually agarose Buffer: salt solution that conducts electricity Well: slots in the gel where samples are loaded

Electric current passed through gel DNA (-) moves toward (+) electrode Small DNA  moves faster Larger DNA  moves slower Use dye to stain DNA for visibility

Carbon fiber electrodes Tools of the trade Gel Tray Micropipette (set to 15 μL) Comb Pipette Tip (disposable!) Carbon fiber electrodes Electrical leads

Casting & Loading a Gel

Using a Micropipette

Loading DNA samples into the wells

Running a Gel + _

DNA Fingerprinting Activity 3 Suspects (labeled #1, #2, #3) 1 Crime Scene Sample (labeled “CS”) #1 #2 #3 CS

Resources Animation: Virtual Labs: http://www.dnalc.org/resources/animations/gelelectrophoresis.html http://www.sumanasinc.com/webcontent/animations/content/gelelectrophoresis.html Virtual Labs: http://learn.genetics.utah.edu/content/labs/gel/ http://www.vivo.colostate.edu/hbooks/genetics/biotech/gels/virgel.html